Construct: ORF TRCN0000468537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005533.1_s317c1
- Derived from:
- ccsbBroadEn_10534
- DNA Barcode:
- CGAATAGACAAAGCCACGTGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTBP1-DT (92070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92070 | CTBP1-DT | CTBP1 divergent transcript | NR_033339.1 | 18.9% | 1_496del;551A>G;1055_2945del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 627
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggttggtg gagtgcacag gcagggacct cactggactt tcctgtctgc 121 tcggcatgga caggcagccc aggagaaggc agcacgtggc cgggtgcagg gacgtaccac 181 ccccacttcc ccaggggagc tggggtcaga cgagtcccag gcactccatc cTCTGCAGCA 241 AGTCAGGTTG TGATTTACTA GGGGGTGGTG AATATAATGG AGAGACTTCT GGGGAGGAAT 301 TCCTGGCTCC CGCGTGGACT TGCAGAGCTC AACAGGCAGC CACGTGGCTG AGTGTCCAGC 361 AAACATCACA TAAGGCTTTG GGTCCTGCAG GTGGGGCTGC CATGAGCAGC AAGCTCAGTC 421 CAGAAGAACA GTTCCTCTCC AGGATCCACT TCCTGCGCAC TTTTATGTGC AGTGTAGCTG 481 GAGCAGAGCT CCCCGGAATT CCACAGGCAA CTGAGAACGG AGAGGGATGC AGGCCAGCCA 541 GGGATCCAGC GTCTTCCCCA TCGTCACTCT CCATGGCCTC CGTCTACACA CAGTGTTCGT 601 CTGCACAGCT TGTCAGCGCG TTATCATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGAATAGA 781 CAAAGCCACG TGGACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt