Construct: ORF TRCN0000468594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001255.1_s317c1
- Derived from:
- ccsbBroadEn_06629
- DNA Barcode:
- GATTGTCCACGCCTCTACTACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEDD9 (4739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_182966.4 | 99.8% | 100% | 141A>G |
| 2 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_006403.4 | 20.4% | 18.8% | (many diffs) |
| 3 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_001142393.1 | 20.1% | 18.4% | (many diffs) |
| 4 | human | 4739 | NEDD9 | neural precursor cell expre... | NR_073131.1 | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtataagaat cttatggcaa gggccttata tgacaatgtc ccagagtgtg 121 ccgaggaact ggcctttcgc aagggagaca tcctgaccgt catagagcag aacacagggg 181 gactggaagg atggtggctg tgctcgttac acggtcggca aggcattgtC CCAGGCAACC 241 GGGTGAAGCT TCTGATTGGT CCCATGCAGG AGACTGCCTC CAGTCACGAG CAGCCTGCCT 301 CTGGACTGAT GCAGCAGACC TTTGGCCAAC AGAAGCTCTA TCAAGTGCCA AACCCACAGG 361 CTGCTCCCCG AGACACCATC TACCAAGTGC CACCTTCCTA CCAAAATCAG GGAATTTACC 421 AAGTCCCCAC TGGCCACGGC ACCCAAGAAC AAGAGGTATA TCAGGTGCCA CCATCAGTGC 481 AGAGAAGCAT TGGGGGAACC AGTGGGCCCC ACGTGGGTAA AAAGGTGTTC CAGAGAGATG 541 GGCAAGTGTC CTATTTCTTA GTGAGAGCCT CTAAACAAAC CAGCTTGTGC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGAGATTGTC CACGCCTCTA CTACAACACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt