Construct: ORF TRCN0000468603
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007877.1_s317c1
- Derived from:
- ccsbBroadEn_06600
- DNA Barcode:
- CGAGGATATGCACGCGCTCCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT3 (4504)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468603
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4504 | MT3 | metallothionein 3 | NM_005954.4 | 99.5% | 98.5% | 191G>C |
| 2 | mouse | 17751 | Mt3 | metallothionein 3 | NM_013603.2 | 88.2% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 217
- ORF end:
- 421
- ORF length:
- 204
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGG ACCCTGAGAC CTGCCCCTGC 241 CCTTCTGGTG GCTCCTGCAC CTGCGCGGAC TCCTGCAAGT GCGAGGGATG CAAATGCACC 301 TCCTGCAAGA AGAGCTGCTG CTCCTGCTGC CCTGCGGAGT GTGAGAAGTG TGCCAAGGAC 361 TGTGTGTGCA AAGGCGGAGA GGCAGCTGAG GCAGAAGCAG AGAAGTCCAG CTGCTGCCAG 421 TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 481 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 541 CTTTATATAT CTTGTGGAAA GGACGACGAG GATATGCACG CGCTCCTAAT ACGCGTTAAG 601 TCgacaatca acctctggat tacaaaattt gtgaaagatt