Construct: ORF TRCN0000468612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002383.1_s317c1
- Derived from:
- ccsbBroadEn_14382
- DNA Barcode:
- TTAACGTATTATTTTTGTATAGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBLCP1 (134510)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134510 | UBLCP1 | ubiquitin like domain conta... | NM_145049.5 | 99.7% | 98.7% | 944_945delAG |
| 2 | mouse | 79560 | Ublcp1 | ubiquitin-like domain conta... | NM_024475.5 | 88% | 96.8% | (many diffs) |
| 3 | mouse | 79560 | Ublcp1 | ubiquitin-like domain conta... | XM_006534522.3 | 88% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctccctatc attgtaaaat ggggtggaca ggagtattca gtgaccacac 121 tttcagaaga tgatactgtg ctcgatctca aacagtttct caagaccctt acaggagttc 181 ttccagaacg ccaaaagtta cttggactca aagttaaagg caaacctgca gaaaatgatg 241 ttaagcttgg agctctcaaa ctgaaaccaa atactaaaat catgatgatg ggaactcgtg 301 aggagagctt ggaagatgtc ttaggtccac cccctgacaa tgatgatgtt gttaatgact 361 ttgatattga agatgaagta gttgaagtag aaaataggga agaaaaccta ctgaaaattt 421 ctcgcagagt gaaagagtac aaagtggaaa ttttgaatcc tcccagggaa gggaaaaagc 481 ttttggtgct agatgttgat tatacattat ttgaccacag gtcttgtgca gagactgggg 541 tagaattaat gcggccatat cttcatgaat ttctaacatc tgcctatgaa gattatgaca 601 ttgttatttg gtctgcaaca aatatgaagt ggattgaagc taaaatgaaa gagctgggag 661 tgagcacaaa tgcaaattat aagattactT TCATGTTGGA TAGTGCTGCT ATGATAACAG 721 TACATACTCC AAGGAGAGGA TTAATAGACG TAAAGCCTCT TGGTGTTATA TGGGGAAAGT 781 TTTCGGAGTT TTACAGCAAG AAAAACACCA TTATGTTTGA TGACATAGGG AGAAATTTTC 841 TAATGAACCC ACAGAATGGA CTAAAGATAA GGCCTTTTAT GAAAGCGCAC CTAAATCGTG 901 ATAAAGACAA AGAACTTTTA AAATTAACTC AGTACCTCAA GGAGATAGCA AAATTAGATG 961 ACTTTTTGGA TCTAAATCAC AAATATTGGG AAAGATATCT CTCAAAGACA AGGACAGTAC 1021 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1081 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1141 TATATATCTT GTGGAAAGGA CGATTAACGT ATTATTTTTG TATAGAAACG CGTTAAGTCg 1201 acaatcaacc tctggattac aaaatttgtg aaagatt