Construct: ORF TRCN0000468683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017852.1_s317c1
- Derived from:
- ccsbBroadEn_12818
- DNA Barcode:
- CCCCGTCTTACGAGATCGTCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF333 (84449)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001300912.2 | 54.6% | 50.1% | (many diffs) |
| 2 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_001352244.2 | 44.7% | 40.9% | (many diffs) |
| 3 | human | 84449 | ZNF333 | zinc finger protein 333 | NM_032433.4 | 25.2% | 22.9% | (many diffs) |
| 4 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_011528362.2 | 25.2% | 22.9% | (many diffs) |
| 5 | human | 84449 | ZNF333 | zinc finger protein 333 | XM_017027367.1 | 25.2% | 22.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atccgtcacc tttgaggatg tggccgtgga gttcatccag gagtgggcat 121 tgctggacag cgcacggagg agcctgtgca aatacaggat gcttgaccag tgcaggaccc 181 tggcctccag gggaacTCCA CCATGCAAAC CCAGTTGTGT CTCCCAGCTG GGGCAAAGAG 241 CAGAGCCAAA GGCAACAGAA CGAGGGATTC TCCGTGCCAC AGGTGTTGCC TGGGAATCTC 301 AACTTAAACC CGAAGAGTTG CCTTCTATGC AGGATCTTTT GGAAGAAGCA TCCTCCAGGG 361 ACATGCAAAT GGGGCCGGGG CTGTTCCTGA GGATGCAGCT GGTGCCCTCC ATAGAAGAGA 421 GGGAGACACC ATTGACTCGA GAGGACCGGC CAGCTCTCCA GGAGCCGCCT TGGTCTCTGG 481 GATGCACGCT GCCTCTCCTG TCCCTGCTGC TGCCATCCTG CCCAGAGCCA CCGTTAGTTC 541 TCCTGGACTC CAGTTCTGCT CCTGACAGGC ACCTGGGCTC CACCCTGGAC ACAGAGGCCA 601 GAGGGATCCC TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC CGTCTTACGA GATCGTCTTT 781 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t