Construct: ORF TRCN0000468741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014968.1_s317c1
- Derived from:
- ccsbBroadEn_03518
- DNA Barcode:
- AATAAATGATTCGCTAAGATCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT57 (55081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55081 | IFT57 | intraflagellar transport 57 | NM_018010.4 | 100% | 100% | |
| 2 | mouse | 73916 | Ift57 | intraflagellar transport 57 | NM_028680.3 | 87.7% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgctgctctg gccgtcgtca cgacgtcggg tttggaagat ggggtgccta 121 ggtcccgtgg cgaagggacc ggggaagtgg tcttggagcg ggggcccggc gcggcctacc 181 acatgttcgt ggtgatggag gacttggtgg agaagctgaa gctgctccgc tacgaggagg 241 agttcctccg gaagagcaac ctgaaggccc cgtccagaca ctattttgca ctgcctacca 301 accctggcga acagttctac atgttttgta ctcttgctgc ttggttgatt aataaagcgg 361 gacgtccctt tgagcagcct caagaatatg atgaccctaa tgcaacaata tctaacatac 421 tatccgagct tcggtcattt ggaagaactg cagattttcc tccttcaaaa ttaaagtcag 481 gttatggaga acatgtatgc tatgttcttg attgcttcgc tgaagaagca ttgaaatata 541 ttggtttcac ctggaaaagg ccaatatacc cagtagaaga attagaagaa gaaagcgttg 601 cagaagatga tgcagaatta acattaaata aagtggatga agaatttgtg gaagaagaga 661 cagataatga agaaaacttt attgatctca acgttttaaa ggcccagaca tatcacttgg 721 atatgaacga gactgccaaa caagaagata ttttggaatc cacaacagat gctgcagaat 781 ggagcctaga agtggaacgt gtactaccgc aactgaaagt cacgattagg actgacaata 841 aggattggag aatccatgtt gaccaaatgc accagcacag aagtggaatt gaaTCTGCTC 901 TAAAGGAGAC CAAGGGATTT TTGGACAAAC TCCATAATGA AATTACTAGG ACTTTGGAAA 961 AGATCAGCAG CCGAGAAAAG TACATCAACA ATCAGCTTGA GAATTTGGTT CAAGAATATC 1021 GTGCAGCTCA AGCCCAGCTG AGTGAGGCAA AGGAGCGATA CCAGCAGGGA AATGGAGGAG 1081 TGACGGAAAG AACCAGACTC CTCTCTGAGG TTATGGAAGA ATTAGAAAAG GTAAAACAAG 1141 AAATGGAAGA AAAGGGCAGC AGCATGACTG ATGGTGCTCC TTTGGTGAAG ATTAAACAGA 1201 GCTTAACAAA ACTGAAGCAA GAAACTGTAG AGATGGACAT TAGAATTGGC ATTGTGGAAC 1261 ACACACTACT CCAATCAAAG CTGAAGGAGA AGTCCAACAT GACTAGGAAC ATGCATGCCA 1321 CAGTTATTCC AGAACCAGCA ACAGGCTTTT ATTATCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GCCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1501 TAAATGATTC GCTAAGATCC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt