Construct: ORF TRCN0000468936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003527.1_s317c1
- Derived from:
- ccsbBroadEn_07549
- DNA Barcode:
- AGATTCCCGGTGCCAAGAGTACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDIL3 (10085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10085 | EDIL3 | EGF like repeats and discoi... | NM_005711.5 | 99.9% | 99.7% | 46G>A |
| 2 | human | 10085 | EDIL3 | EGF like repeats and discoi... | NM_001278642.1 | 97.8% | 97.7% | 46G>A;195_196ins30 |
| 3 | mouse | 13612 | Edil3 | EGF-like repeats and discoi... | NM_001037987.3 | 89.7% | 95.4% | (many diffs) |
| 4 | mouse | 13612 | Edil3 | EGF-like repeats and discoi... | NM_010103.4 | 87.8% | 93.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcgctcggta gccgtctggc tcttggtcgg gctcagcctc agtgtccccc 121 agttcggcaa aggtgatatt tgtgatccca atccatgtga aaatggaggt atctgtttgc 181 caggattggc tgatggttcc ttttcctgtg agtgtccaga tggcttcaca gaccccaact 241 gttctagtgt tgtggaggtt gcatcagatg aagaagaacc aacttcagca ggtccctgca 301 ctcctaatcc atgccataat ggaggaacct gtgaaataag tgaagcatac cgaggggata 361 cattcatagg ctatgtttgt aaatgtcccc gaggatttaa tgggattcac tgtcagcaca 421 acataaatga atgcgaagtt gagccttgca aaaatggtgg aatatgtaca gatcttgttg 481 ctaactattc ctgtgagtgc ccaggcgaat ttatgggaag aaattgtcaa tacaaatgct 541 caggcccact gggaattgaa ggtggaatta tatcaaacca gcaaatcaca gcttcctcta 601 ctcaccgagc tctttttgga ctccaaaaat ggtatcccta ctatgcacgt cttaataaga 661 aggggcttat aaatgcgtgg acagctgcag aaaatgacag atggccgtgg attcagataa 721 atttgcaaag gaaaatgaga gttactggtg tgattaccca aggagccaag aggattggaa 781 gcccagagta tataaaatcc tacaaaattg cctacagtaa tgatggaaag acttgggcaa 841 tgtacaaagt gaaaggcacc aatgaagaca tggtgtttcg tggaaacatt gataacaaca 901 ctccatatgc taactctttc acacccccca taaaagctca gtatgtaaga ctctatcccc 961 aagtttgtcg aagacattgc actttgcgaa tggaacttct tggctgtgaa ctgtcgggtt 1021 gttctgagcc tctgggtatg aaatcaggac atatacaaga ctatcagatc actgccTCCA 1081 GCATCTTCAG AACGCTCAAC ATGGACATGT TCACTTGGGA ACCAAGGAAA GCTCGGCTGG 1141 ACAAGCAAGG CAAAGTGAAT GCCTGGACCT CTGGCCACAA TGACCAGTCA CAATGGTTAC 1201 AGGTGGATCT TCTTGTTCCA ACCAAAGTGA CTGGCATCAT TACACAAGGA GCTAAAGATT 1261 TTGGTCATGT ACAGTTTGTT GGCTCCTACA AACTGGCTTA CAGCAATGAT GGAGAACACT 1321 GGACTGTATA CCAGGATGAA AAGCAAAGAA AAGATAAGGT TTTCCAGGGA AATTTTGACA 1381 ATGACACTCA CAGAAAAAAT GTCATCGACC CTCCCATCTA TGCACGACAC ATAAGAATCC 1441 TTCCTTGGTC CTGGTACGGG AGGATCACAT TGCGGTCAGA GCTGCTGGGC TGCACAGAGG 1501 AGGAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGATTCCCG GTGCCAAGAG TACTGACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt