Construct: ORF TRCN0000469057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013007.1_s317c1
- Derived from:
- ccsbBroadEn_08830
- DNA Barcode:
- CATGTCCGTCACACAATGCCCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAL3ST2 (64090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64090 | GAL3ST2 | galactose-3-O-sulfotransfer... | NM_022134.3 | 99.8% | 99.7% | 10A>T;447C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgtccttg ctgggcggct tgcagagata cttccgggtc atcctcctcc 121 tcctcctggc cctgactctg ctcctgctgg ccggattcct gcactcggac ttagagctgg 181 acacacccct gtttgggggc caggctgagg ggccgccggt caccaacatc atgttcctga 241 agacgcacaa gacggccagc agcacggtgc tcaacatcct ctaccgcttc gccgagaccc 301 acaacctgtc cgtggcgctg cccgccggct cacgcgtcca cctgggctac ccctggctct 361 tcctggcgcg ctacgtggaa ggcgtggggt cgcagcagcg cttcaacatc atgtgcaacc 421 acctgaggtt caacctgcct caggtgcaga aagtcatgcc caacgacacc ttctacttct 481 ccatcctgag gaaccccgtg ttccagctgg agtcttcctt catctactac aaaacctacg 541 cccccgcctt ccggggcgcc ccgagcctgg acgcgttcct ggcctcgccg cggacgttct 601 acaacgacag ccgccacctc aggaacgtct acgccaagaa caacatgtgg ttcgacttcg 661 gcttcgaccc caacgcgcag tgcgaggagg gctacgtgcg cgcgcgcatc gccgaggtgg 721 agcggcgctt ccggctggtg ctcatcgccg agcacctgga cgagtccctg gtgctgctgc 781 ggcgccggct gcgctgggcg ctggacgacg tggtggcctt caggctcaac tcccgcagcg 841 cgcgctccgt ggcccgcctg tcgcccgaga cccgggagcg cgcgcggagc tggtgcgcgc 901 tggactggcg cctgtacgag catttcaacc gcacccTCTG GGCGCAGCTG CGCGCCGAGC 961 TGGGGCCGCG GCGGCTGCGC GGGGAGGTGG AGCGGCTGCG CGCCCGGAGG CGCGAACTCG 1021 CGAGCCTGTG CCTGCAGGAC GGCGGCGCGC TCAAGAACCA CACGCAGATC AGAGACCCGC 1081 GCCTGCGCCC CTACCAGTCC GGCAAGGCCG ACATCCTGGG TTACAACCTC CGGCCGGGCC 1141 TGGACAACCA GACGCTGGGC GTGTGCCAGA GGCTTGTGAT GCCTGAGCTC CAGTACATGG 1201 CCCGCCTGTA CGCCCTGCAG TTCCCGGAGA AGCCCCTCAA GAACATCCCG TTCCTGGGGG 1261 CGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGACA TGTCCGTCAC ACAATGCCCG GGACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt