Construct: ORF TRCN0000469073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008432.1_s317c1
- Derived from:
- ccsbBroadEn_01072
- DNA Barcode:
- CGACCCTGAACCGTATAGAACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFB6 (4712)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_002493.5 | 100% | 100% | |
| 2 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_182739.3 | 88.2% | 88.2% | 271_272ins45 |
| 3 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_001199987.1 | 75.7% | 75.7% | 180_181ins93 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 463
- ORF length:
- 384
- Sequence:
-
1 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT 61 TTGTACAAAA AAGTTGGCAT GACGGGGTAC ACTCCGGATG AGAAACTGCG GCTGCAGCAG 121 CTGCGAGAGC TGAGAAGGCG ATGGCTGAAG GACCAGGAGC TGAGCCCTCG GGAGCCGGTG 181 CTGCCCCCAC AGAAGATGGG GCCTATGGAG AAATTCTGGA ATAAATTTTT GGAGAATAAA 241 TCCCCTTGGA GGAAAATGGT CCATGGGGTA TACAAAAAGA GTATCTTTGT TTTCACTCAT 301 GTACTTGTAC CTGTCTGGAT TATTCATTAT TACATGAAGT ATCATGTTTC TGAAAAACCA 361 TATGGCATAG TTGAAAAGAA GTCCAGAATA TTCCCTGGTG ATACAATTCT GGAGACTGGA 421 GAAGTAATTC CACCAATGAA AGAATTTCCT GATCAACATC ATTACCCAAC TTTCTTGTAC 481 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 541 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 601 AAGGACGACG ACCCTGAACC GTATAGAACT GCACGCGTTA AGTCgacaat caacctctgg 661 attacaaaat ttgtgaaaga tt