Construct: ORF TRCN0000469086
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005341.1_s317c1
- Derived from:
- ccsbBroadEn_05818
- DNA Barcode:
- ACATTTGCCCACGATTACACCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A5 (292)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469086
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 292 | SLC25A5 | solute carrier family 25 me... | NM_001152.5 | 99.7% | 99.6% | 332T>G;699T>C |
| 2 | human | 293 | SLC25A6 | solute carrier family 25 me... | NM_001636.4 | 78.7% | 92.2% | (many diffs) |
| 3 | mouse | 11740 | Slc25a5 | solute carrier family 25 (m... | NM_007451.4 | 92.7% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agatgccgct gtgtccttcg ccaaggactt cctggcaggt ggagtggccg 121 cagccatctc caagacggcg gtagcgccca tcgagcgggt caagctgctg ctgcaggtgc 181 agcatgccag caagcagatc actgcagata agcaatacaa aggcattata gactgcgtgg 241 tccgtattcc caaggagcag ggagttctgt ccttctggcg cggtaacctg gccaatgtca 301 tcagatactt ccccacccag gctcttaact tcgccttcaa agataaatac aagcagatct 361 tcctgggtgg tgtggacaag agaacccagt tttggcgcta ctttgcaggg aatctggcat 421 cgggtggtgc cgcaggggcc acatccctgt gttttgtgta ccctcttgat tttgcccgta 481 cccgtctagc agctgatgtg ggtaaagctG GAGCTGAAAG GGAATTCCGA GGCCTCGGTG 541 ACTGCCTGGT TAAGATCTAC AAATCTGATG GGATTAAGGG CCTGTACCAA GGCTTTAACG 601 TGTCTGTGCA GGGTATTATC ATCTACCGAG CCGCCTACTT CGGTATCTAT GACACTGCAA 661 AGGGAATGCT TCCGGATCCC AAGAACACTC ACATCGTCAT CAGCTGGATG ATCGCACAGA 721 CTGTCACTGC TGTTGCCGGG TTGACTTCCT ATCCATTTGA CACCGTTCGC CGCCGCATGA 781 TGATGCAGTC AGGGCGCAAA GGAACTGACA TCATGTACAC AGGCACGCTT GACTGCTGGC 841 GGAAGATTGC TCGTGATGAA GGAGGCAAAG CTTTTTTCAA GGGTGCATGG TCCAATGTTC 901 TCAGAGGCAT GGGTGGTGCT TTTGTGCTTG TCTTGTATGA TGAAATCAAG AAGTACACAT 961 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAACATT TGCCCACGAT TACACCCAAA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt