Construct: ORF TRCN0000469112

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010542.3_s317c1
Derived from:
ccsbBroadEn_14584
DNA Barcode:
ATCATCCATTTTGTGAGGGCATGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKB (1152)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469112
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1152 CKB creatine kinase B NM_001823.5 99.8% 100% 456C>A;1092G>A
2 human 1152 CKB creatine kinase B NM_001362531.2 93.9% 94% 346_417del;528C>A;1164G>A
3 human 1152 CKB creatine kinase B XM_017020952.1 56.8% 56.9% 456C>A;651_652ins492
4 mouse 12709 Ckb creatine kinase, brain NM_021273.4 90.4% 96.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1209
ORF length:
1143
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc cttctccaac agccacaacg cactgaagct gcgcttcccg gccgaggacg
121agttccccga cctgagcgcc cacaacaacc acatggccaa ggtgctgacc cccgagctgt
181acgcggagct gcgcgccaag agcacgccga gcggcttcac gctggacgac gtcatccaga
241caggcgtgga caacccgggc cacccgtaca tcatgaccgt gggctgcgtg gcgggcgacg
301aggagtccta cgaagtgttc aaggatctct tcgaccccat catcgaggac cggcacggcg
361gctacaagcc cagcgatgag cacaagaccg acctcaaccc cgacaacctg cagggcggcg
421acgacctgga ccccaactac gtgctgagct cgcgggtgcg cacgggccgc agcatccgtg
481gcttctgcct ccccccgcac tgcagccgcg gggagcgccg agccatcgag aagctcgcgg
541tggaagccct gtccagcctg gacggcgacc tggcgggccg atactacgcg ctcaagagca
601tgacggaggc ggagcagcag cagctcatcg acgaccactt cctcttcgac aagcccgtgt
661cgcccctgct gctggcctcg ggcatggccc gcgactggcc cgacgcccgc ggtatctggc
721acaatgacaa taagaccttc ctggtgtggg tcaacgagga ggaccaccTG CGGGTCATCT
781CCATGCAGAA GGGGGGCAAC ATGAAGGAGG TGTTCACCCG CTTCTGCACC GGCCTCACCC
841AGATTGAAAC TCTCTTCAAG TCTAAGGACT ATGAGTTCAT GTGGAACCCT CACCTGGGCT
901ACATCCTCAC CTGCCCATCC AACCTGGGCA CCGGGCTGCG GGCAGGTGTG CATATCAAGC
961TGCCCAACCT GGGCAAGCAT GAGAAGTTCT CGGAGGTGCT TAAGCGGCTG CGACTTCAGA
1021AGCGAGGCAC AGGCGGTGTG GACACGGCTG CGGTGGGCGG GGTCTTCGAC GTCTCCAACG
1081CTGACCGCCT GGGCTTCTCA GAGGTGGAGC TGGTGCAGAT GGTGGTGGAC GGAGTGAAGC
1141TGCTCATCGA GATGGAACAG CGGCTGGAGC AGGGCCAGGC CATCGACGAC CTCATGCCTG
1201CCCAGAAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1261CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1321TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCATC CATTTTGTGA GGGCATGGAC
1381GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)