Construct: ORF TRCN0000469112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010542.3_s317c1
- Derived from:
- ccsbBroadEn_14584
- DNA Barcode:
- ATCATCCATTTTGTGAGGGCATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CKB (1152)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1152 | CKB | creatine kinase B | NM_001823.5 | 99.8% | 100% | 456C>A;1092G>A |
| 2 | human | 1152 | CKB | creatine kinase B | NM_001362531.2 | 93.9% | 94% | 346_417del;528C>A;1164G>A |
| 3 | human | 1152 | CKB | creatine kinase B | XM_017020952.1 | 56.8% | 56.9% | 456C>A;651_652ins492 |
| 4 | mouse | 12709 | Ckb | creatine kinase, brain | NM_021273.4 | 90.4% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cttctccaac agccacaacg cactgaagct gcgcttcccg gccgaggacg 121 agttccccga cctgagcgcc cacaacaacc acatggccaa ggtgctgacc cccgagctgt 181 acgcggagct gcgcgccaag agcacgccga gcggcttcac gctggacgac gtcatccaga 241 caggcgtgga caacccgggc cacccgtaca tcatgaccgt gggctgcgtg gcgggcgacg 301 aggagtccta cgaagtgttc aaggatctct tcgaccccat catcgaggac cggcacggcg 361 gctacaagcc cagcgatgag cacaagaccg acctcaaccc cgacaacctg cagggcggcg 421 acgacctgga ccccaactac gtgctgagct cgcgggtgcg cacgggccgc agcatccgtg 481 gcttctgcct ccccccgcac tgcagccgcg gggagcgccg agccatcgag aagctcgcgg 541 tggaagccct gtccagcctg gacggcgacc tggcgggccg atactacgcg ctcaagagca 601 tgacggaggc ggagcagcag cagctcatcg acgaccactt cctcttcgac aagcccgtgt 661 cgcccctgct gctggcctcg ggcatggccc gcgactggcc cgacgcccgc ggtatctggc 721 acaatgacaa taagaccttc ctggtgtggg tcaacgagga ggaccaccTG CGGGTCATCT 781 CCATGCAGAA GGGGGGCAAC ATGAAGGAGG TGTTCACCCG CTTCTGCACC GGCCTCACCC 841 AGATTGAAAC TCTCTTCAAG TCTAAGGACT ATGAGTTCAT GTGGAACCCT CACCTGGGCT 901 ACATCCTCAC CTGCCCATCC AACCTGGGCA CCGGGCTGCG GGCAGGTGTG CATATCAAGC 961 TGCCCAACCT GGGCAAGCAT GAGAAGTTCT CGGAGGTGCT TAAGCGGCTG CGACTTCAGA 1021 AGCGAGGCAC AGGCGGTGTG GACACGGCTG CGGTGGGCGG GGTCTTCGAC GTCTCCAACG 1081 CTGACCGCCT GGGCTTCTCA GAGGTGGAGC TGGTGCAGAT GGTGGTGGAC GGAGTGAAGC 1141 TGCTCATCGA GATGGAACAG CGGCTGGAGC AGGGCCAGGC CATCGACGAC CTCATGCCTG 1201 CCCAGAAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCATC CATTTTGTGA GGGCATGGAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt