Construct: ORF TRCN0000469163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003156.1_s317c1
- Derived from:
- ccsbBroadEn_06616
- DNA Barcode:
- ACCACTTCCCCATCAATTTGAGAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NARS1 (4677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4677 | NARS1 | asparaginyl-tRNA synthetase 1 | NM_004539.4 | 99.8% | 99.8% | 1643_1644delCAinsA |
| 2 | human | 4677 | NARS1 | asparaginyl-tRNA synthetase 1 | XM_005266700.2 | 99.6% | 99.6% | 8_9insAGC;1640_1641delCAinsA |
| 3 | mouse | 70223 | Nars | asparaginyl-tRNA synthetase | NM_001142950.1 | 84.9% | 91.5% | (many diffs) |
| 4 | mouse | 70223 | Nars | asparaginyl-tRNA synthetase | NM_027350.3 | 84.8% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1710
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctagcagag ctgtacgtct ctgaccgaga gggaagcgat gccacgggag 121 atggaaccaa ggagaaacca tttaaaacag gtctaaaggc tttgatgaca gtagggaaag 181 aaccatttcc taccatttac gtagattcac aaaaagaaaa tgagaggtgg aatgttattt 241 ctaaatcaca gttgaagaac attaaaaaga tgtggcatag ggaacaaatg aagagtgaat 301 cccgggaaaa gaaagaggca gaagatagtt tacgaagaga aaagaacctg gaagaagcaa 361 agaagattac cattaaaaat gatccaagtc tcccagagcc aaaatgtgtg aagattggtg 421 cgttagaagg atatagaggc caaagagtaa aggtgtttgg ctgggtccac aggctgcgca 481 ggcaaggaaa gaatttaatg tttctggtgt tgcgagatgg tacaggttat cttcagtgtg 541 tcttggcgga tgagttgtgt cagtgctaca atggagttct cttgtccacg gagagcagtg 601 ttgcagtgta tggaatgcta aatcttaccc caaagggcaa gcaggctcca ggtggccatg 661 agctgagttg tgacttctgg gaactaattg ggttggcccc tgctggagga gctgacaacc 721 tgatcaatga ggagtctgac gttgatgtcc agctcaacaa cagacacatg atgatccgag 781 gagaaaacat gtccaaaatc ctaaaagcac gatccatggt caccaggtgc tttagagatc 841 acttctttga tagggggtac tatgaagtta ctcctccaac attagtgcaa acacaagtag 901 aaggtggtgc cacactcttc aagcttgact attttgggga agaggcattt ttgactcaat 961 cctctcagtt gtacttggag acctgcctcc cagccctggg agatgttttt tgtattgctc 1021 agtcataccg ggcagagcag tccagaacac gaaggcacct ggctgagtac actcacgtgg 1081 aagctgagtg tcctttcctg acttttgacg acctcctgaa ccggttggag gacttggttt 1141 gtgatgtggt agatcgaata ttgaagtcac ctgcagggag catagtgcat gagctcaacc 1201 cgaactttca gccccccaaa cggcctttca aacggatgaa ctattcagat gctatcgttt 1261 ggctaaaaga acatgatgta aagaaagaag atggaacttt ctatgaattt ggagaagata 1321 tcccagaagc tcctgagaga ctgatgacag acaccattaa tgaaccaatc ttgctgtgtc 1381 gatttCCTGT GGAGATCAAG TCCTTCTACA TGCAGCGATG TCCTGAGGAT TCCCGTCTTA 1441 CTGAATCTGT CGACGTGTTG ATGCCCAATG TTGGTGAGAT TGTGGGAGGC TCAATGCGTA 1501 TCTTTGATAG TGAAGAAATA CTGGCAGGTT ATAAAAGGGA AGGGATTGAC CCCACTCCCT 1561 ATTACTGGTA TACGGATCAG AGAAAATACG GTACATGTCC CCATGGAGGA TATGGCTTGG 1621 GCTTGGAACG ATTCTTAACG TGGATTCTGA ATAGGTATCA CATCCGAGAC GTGTGCTTAT 1681 ACCCTCGATT TGTCCAGCGT TGCACGCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT 1741 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 1801 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCACT 1861 TCCCCATCAA TTTGAGATAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1921 gaaagatt