Construct: ORF TRCN0000469168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013014.1_s317c1
- Derived from:
- ccsbBroadEn_06596
- DNA Barcode:
- AGCTTAAGTGGCATACTTGCTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSR1 (4481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469168
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4481 | MSR1 | macrophage scavenger recept... | NM_138715.3 | 99.9% | 100% | 1086G>A |
| 2 | human | 4481 | MSR1 | macrophage scavenger recept... | NM_001363744.1 | 96% | 96.1% | 1_54del;1140G>A |
| 3 | human | 4481 | MSR1 | macrophage scavenger recept... | NM_138716.2 | 86% | 85.8% | 1033_1034ins189 |
| 4 | human | 4481 | MSR1 | macrophage scavenger recept... | NM_002445.4 | 77.9% | 77% | (many diffs) |
| 5 | human | 4481 | MSR1 | macrophage scavenger recept... | XM_024447161.1 | 74.9% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagtgggat cactttcaca atcaacagga ggacactgat agctgctccg 121 aatctgtgaa atttgatgct cgctcaatga cagctttgct tcctccgaat cctaaaaaca 181 gcccttccct tcaagagaaa ctgaagtcct tcaaagctgc actgattgcc ctttacctcc 241 tcgtgtttgc agttctcatc cctctcattg gaatagtggc agctcaactc ctgaagtggg 301 aaacgaagaa ttgctcagtt agttcaacta atgcaaatga tataactcaa agtctcacgg 361 gaaaaggaaa tgacagcgaa gaggaaatga gatttcaaga agtctttatg gaacacatga 421 gcaacatgga gaagagaatc cagcatattt tagacatgga agccaacctc atggacacag 481 agcatttcca aaatttcagc atgacaactg atcaaagatt taatgacatt cttctgcagc 541 taagtacctt gttttcctca gtccagggac atgggaatgc aatagatgaa atctccaagt 601 ccttaataag tttgaatacc acattgcttg atttgcagct caacatagaa aatctgaatg 661 gcaaaatcca agagaatacc ttcaaacaac aagaggaaat cagtaaatta gaggagcgtg 721 tttacaatgt atcagcagaa attatggcta tgaaagaaga acaagtgcat ttggaacagg 781 aaataaaagg agaagtgaaa gtactgaata acatcactaa tgatctcaga ctgaaagatt 841 gggaacattc tcagaccttg agaaatatca ctttaattca aggtcctcct ggacccccgg 901 gtgaaaaagg agatcgaggt cccactggag aaagtggtcc acgaggattt ccaggtccaa 961 taggtcctcc gggtcttaaa ggtgatcggg gagcaattgg ctttcctgga agtcgaggac 1021 tcccaggata tgccggaagg ccaggaaatt ctggaccaaa aggCCAGAAA GGGGAAAAGG 1081 GGAGTGGAAA CACATTAACT CCATTTACGA AAGTTCGACT GGTCGGTGGG AGCGGCCCTC 1141 ACGAGGGGAG AGTGGAGATA CTCCACAGCG GCCAGTGGGG TACAATTTGT GACGATCGCT 1201 GGGAAGTGCG CGTTGGACAG GTCGTCTGTA GGAGCTTGGG ATACCCAGGT GTTCAAGCCG 1261 TGCACAAGGC AGCTCACTTT GGACAAGGTA CTGGTCCAAT ATGGCTGAAT GAAGTGTTTT 1321 GTTTTGGGAG AGAATCATCT ATTGAAGAAT GTAAAATTCG GCAATGGGGG ACAAGAGCCT 1381 GTTCACATTC TGAAGATGCT GGAGTCACTT GCACTTTATA CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGAAGCTTA AGTGGCATAC TTGCTGCAAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt