Construct: ORF TRCN0000469176
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013533.1_s317c1
- Derived from:
- ccsbBroadEn_00060
- DNA Barcode:
- TATTCGCGGCCAACGACCGCGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMBP (259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469176
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259 | AMBP | alpha-1-microglobulin/bikun... | NM_001633.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gagcctcggg gccctgctct tgctgctgag cgcctgcctg gcggtgagcg 121 ctggccctgt gccaacgccg cccgacaaca tccaagtgca ggaaaacttc aatatctctc 181 ggatctatgg gaagtggtac aacctggcca tcggttccac ctgcccctgg ctgaagaaga 241 tcatggacag gatgacagtg agcacgctgg tgctgggaga gggcgctaca gaggcggaga 301 tcagcatgac cagcactcgt tggcggaaag gtgtctgtga ggagacgtct ggagcttatg 361 agaaaacaga tactgatggg aagtttctct atcacaaatc caaatggaac ataaccatgg 421 agtcctatgt ggtccacacc aactatgatg agtatgccat tttcctgacc aagaaattca 481 gccgccatca tggacccacc attactgcca agctctacgg gcgggcgccg cagctgaggg 541 aaactctcct gcaggacttc agagtggttg cccagggtgt gggcatccct gaggactcca 601 tcttcaccat ggctgaccga ggtgaatgtg tccctgggga gcaggaacca gagcccatct 661 taatcccgag agtccggagg gctgtgctac cccaagaaga ggaaggatca gggggtgggc 721 aactggtaac tgaagtcacc aagaaagaag attcctgcca gctgggctac tcggccggtc 781 cctgcatggg aatgaccagc aggtatttct ataatggtac atccatggcc tgtgagactt 841 tccagtacgg cggctgcatg ggcaacggta acaacttcGT CACAGAAAAG GAGTGTCTGC 901 AGACCTGCCG AACTGTGGCG GCCTGCAATC TCCCCATAGT CCGGGGCCCC TGCCGAGCCT 961 TCATCCAGCT CTGGGCATTT GATGCTGTCA AGGGGAAGTG CGTCCTCTTC CCCTACGGGG 1021 GCTGCCAGGG CAACGGGAAC AAGTTCTACT CAGAGAAGGA GTGCAGAGAG TACTGCGGTG 1081 TCCCTGGTGA TGGTGATGAG GAGCTGCTGC GCTTCTCCAA CTGCCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATAT TCGCGGCCAA CGACCGCGCT TACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t