Construct: ORF TRCN0000469184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015903.1_s317c1
- Derived from:
- ccsbBroadEn_07077
- DNA Barcode:
- GTGATGCCGTGCAAATGCGACCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN8 (7103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7103 | TSPAN8 | tetraspanin 8 | NM_001369760.1 | 99.5% | 98.7% | 103G>A;218G>C;637T>G |
| 2 | human | 7103 | TSPAN8 | tetraspanin 8 | NM_004616.3 | 99.5% | 98.7% | 103G>A;218G>C;637T>G |
| 3 | human | 7103 | TSPAN8 | tetraspanin 8 | XM_006719583.3 | 99.5% | 98.7% | 103G>A;218G>C;637T>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggtgtgagt gcctgtataa aatattctat gtttaccttc aacttcttgt 121 tctggctatg tggtatcttg atcctagcat tagcaatatg ggtacgaata agcaatgact 181 ctcaagcaat ttttggttct gaagatgtag gctctagctc ctacgttgct gtggacatat 241 tgattgctgt aggtgccatc atcatgattc tgggcttcct ggcatgctgc ggtgctataa 301 aagaaagtcg ctgcatgctt ctgttgTTTT TCATAGGCTT GCTTCTGATC CTGCTCCTGC 361 AGGTGGCGAC AGGTATCCTA GGAGCTGTTT TCAAATCTAA GTCTGATCGC ATTGTGAATG 421 AAACTCTCTA TGAAAACACA AAGCTTTTGA GCGCCACAGG GGAAAGTGAA AAACAATTCC 481 AGGAAGCCAT AATTGTGTTT CAAGAAGAGT TTAAATGCTG CGGTTTGGTC AATGGAGCTG 541 CTGATTGGGG AAATAATTTT CAACACTATC CTGAATTATG TGCCTGTCTA GATAAGCAGA 601 GACCATGCCA AAGCTATAAT GGAAAACAAG TTTACAAAGA GACCTGTATT TCTTTCATAA 661 AAGACTTCTT GGCAAAAAAT TTGATTATAG TTATTGGAAT AGCATTTGGA CTGGCAGTTA 721 TTGAGATACT GGGTTTGGTG TTTTCTATGG TCCTGTATTG CCAGATCGGG AACAAATGCC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAGTGATGCC GTGCAAATGC GACCATACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt