Construct: ORF TRCN0000469232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011965.1_s317c1
- Derived from:
- ccsbBroadEn_01496
- DNA Barcode:
- TGTACTTGGATAGATGCAGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL3L1 (6349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414062 | CCL3L3 | C-C motif chemokine ligand ... | NM_001001437.4 | 100% | 100% | |
| 2 | human | 6349 | CCL3L1 | C-C motif chemokine ligand ... | NM_021006.5 | 100% | 100% | |
| 3 | human | 6348 | CCL3 | C-C motif chemokine ligand 3 | NM_002983.3 | 95.6% | 94.6% | (many diffs) |
| 4 | human | 6349 | CCL3L1 | C-C motif chemokine ligand ... | NR_111964.1 | 27.9% | 1_85del;162_353del;557_997del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 369
- ORF length:
- 279
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCAGGTCTC CACTGCTGCC CTTGCCGTCC 121 TCCTCTGCAC CATGGCTCTC TGCAACCAGG TCCTCTCTGC ACCACTTGCT GCTGACACGC 181 CGACCGCCTG CTGCTTCAGC TACACCTCCC GACAGATTCC ACAGAATTTC ATAGCTGACT 241 ACTTTGAGAC GAGCAGCCAG TGCTCCAAGC CCAGTGTCAT CTTCCTAACC AAGAGAGGCC 301 GGCAGGTCTG TGCTGACCCC AGTGAGGAGT GGGTCCAGAA ATACGTCAGT GACCTGGAGC 361 TGAGTGCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTACT TGGATAGATG CAGACCCCAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt