Construct: ORF TRCN0000469274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012607.1_s317c1
- Derived from:
- ccsbBroadEn_15866
- DNA Barcode:
- CCTCTTCTTTCCCTTTGTGTTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ING3 (54556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54556 | ING3 | inhibitor of growth family ... | XM_017012369.2 | 97.1% | 95.6% | 268C>G;269_270insT;273_273delCinsTGGAAT |
| 2 | human | 54556 | ING3 | inhibitor of growth family ... | NM_198267.2 | 95% | 95.6% | 268C>G;270_274delGCACT;276_277insTTTGGAAT |
| 3 | human | 54556 | ING3 | inhibitor of growth family ... | NM_019071.3 | 22% | 21.2% | (many diffs) |
| 4 | human | 54556 | ING3 | inhibitor of growth family ... | XR_001744819.1 | 13.3% | 1_112del;392_2085del | |
| 5 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_001311061.1 | 20.9% | 21.7% | (many diffs) |
| 6 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_023626.4 | 20.4% | 21.1% | (many diffs) |
| 7 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_006505166.3 | 15.1% | 15.4% | (many diffs) |
| 8 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_017321747.1 | 12.6% | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 369
- ORF length:
- 279
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTTGTACCT AGAAGACTAT CTGGAAATGA 121 TTGAGCAGCT TCCTATGGAT CTGCGGGACC GCTTCACGGA AATGCGCGAG ATGGACCTGC 181 AGGTGCAGAA TGCAATGGAT CAACTAGAAC AAAGAGTCAG TGAATTCTTT ATGAATGCAA 241 AGAAAAATAA ACCTGAGTGG AGGGAAGAGC AAATGGCATC CATCAAAAAA GACTACTATA 301 AAGCTTTGGA AGATGCAGAT GAGAAGGTTC AGTTGGCAAA CCAGATATAT GACTTGGATC 361 TTTGGAATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTCTT CTTTCCCTTT GTGTTTTGAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt