Construct: ORF TRCN0000469352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012986.1_s317c1
- Derived from:
- ccsbBroadEn_16035
- DNA Barcode:
- CCACAGGTAACTATGTCTTATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYSTM1 (84418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84418 | CYSTM1 | cysteine rich transmembrane... | NM_032412.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 357
- ORF length:
- 291
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccaagagaac cctccaccat atccaggccc tggtccaacg gccccatacc 121 caccttatcc accacaacca atgggtccag gacctatggg gggacccTAC CCACCTCCTC 181 AAGGGTACCC CTACCAAGGA TACCCACAGT ACGGCTGGCA GGGTGGACCT CAGGAGCCTC 241 CTAAAACCAC AGTGTATGTG GTAGAAGACC AAAGAAGAGA TGAGCTAGGA CCATCCACCT 301 GCCTCACAGC CTGCTGGACG GCTCTCTGTT GCTGCTGTCT CTGGGACATG CTCACCTGCC 361 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 421 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 481 ATATATCTTG TGGAAAGGAC GACCACAGGT AACTATGTCT TATGTCACGC GTTAAGTCga 541 caatcaacct ctggattaca aaatttgtga aagatt