Construct: ORF TRCN0000469364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015401.1_s317c1
- Derived from:
- ccsbBroadEn_08096
- DNA Barcode:
- ATTATACCCAGATACCACACACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS18B (28973)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28973 | MRPS18B | mitochondrial ribosomal pro... | NM_014046.4 | 99.8% | 99.6% | 586G>A |
| 2 | human | 28973 | MRPS18B | mitochondrial ribosomal pro... | XM_024446408.1 | 35.7% | 35% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtctgta ttaaacaccg tgctgaggcg gcttcctatg ctatctctct 121 tccgaggttc tcacagagtt caggttcccc tccagactct ttgcaccaaa gctccctctg 181 aggaagattc tttgtcctca gttcccattt ctccttataa ggatgagccc tggaaatatc 241 tggaatcaga agaataccag gagcgatatg gttctcgccc cgtctgggct gactaccgcc 301 gcaaccacaa gggtggtgta cccccacagc ggactcggaa gacatgtatt cgtcggaata 361 aagttgttgg gaatccctgc cccatctgtc gagatcacaa gttgcatgtt gactttagga 421 acgtgaagct cttggagcaa tttGTCTGCG CCCACACGGG TATCATCTTC TATGCTCCAT 481 ACACAGGAGT CTGTGTGAAG CAGCACAAGC GGTTGACCCA GGCCATCCAG AAAGCCAGGG 541 ATCATGGTCT CCTCATTTAC CACATCCCCC AGGTTGAACC ACGGGACCTT GACTTCAGTA 601 CCTCTCATGG GGCTGTGAGT GCTACTCCGC CAGCCCCCAC CCTGGTCTCA AGTGACCCCT 661 GGTACCCATG GTACAACTGG AAACAGCCAC CGGAGAGAGA ACTGTCTCGC CTTCGCCGGC 721 TTTACCAGGG TCATCTCCAA GAAGAGAGTG GCCCCCCACC TGAGTCAATG CCCAAGATGC 781 CCCCTAGAAC ACCAGCGGAA GCCTCCTCCA CTGGGCAGAC AGGCCCTCAG AGTGCTCTGT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAATTAT ACCCAGATAC CACACACATA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt