Construct: ORF TRCN0000469387

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000406.1_s317c1
Derived from:
ccsbBroadEn_00888
DNA Barcode:
GCCCATGACCTAGTATACAAAAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ITPK1 (3705)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469387
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3705 ITPK1 inositol-tetrakisphosphate ... NM_001142594.3 100% 100%
2 human 3705 ITPK1 inositol-tetrakisphosphate ... NM_001142593.3 73.9% 72.6% (many diffs)
3 human 3705 ITPK1 inositol-tetrakisphosphate ... NM_014216.6 73.9% 72.6% (many diffs)
4 human 3705 ITPK1 inositol-tetrakisphosphate ... XM_017021262.1 60.2% 56.5% (many diffs)
5 human 3705 ITPK1 inositol-tetrakisphosphate ... NM_001363707.1 45.3% 44.2% (many diffs)
6 human 3705 ITPK1 inositol-tetrakisphosphate ... XM_017021263.1 42.7% 41.6% (many diffs)
7 human 3705 ITPK1 inositol-tetrakisphosphate ... XM_017021264.1 42.7% 41.6% (many diffs)
8 mouse 217837 Itpk1 inositol 1,3,4-triphosphate... NM_172584.3 65.3% 70.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1008
ORF length:
942
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gacctttctg aaagggaaga gagttggcta ctggctgagc gagaagaaaa
121tcaagaagct gaatttccag gccttcgccg agctgtgcag gaagcgaggg atggaggttg
181tgcagctgaa ccttagccgg ccgatcgagg agcagggccc cctggacgtc atcatccaca
241agctgactga cgtcatcctt gaagccgacc agaatgatag ccagtccctg gagctggtgc
301acaggttcca ggagtacatc gatgcccacc ctgagaccat cgtcctggac ccgctccctg
361ccatcagaac cctgcttgac cgctccaagt cctatgagct catccggaag attgaggcct
421acatggaaga cgacaggatc tgctcgccac ccttcatgga gctcacgagc ctgtgcgggg
481atgacaccat gcggctgctg gagaagaacg gcttgacttt cccattcatt tgcaaaacca
541gagtggctca tggcaccaac tctcacgaga tggctatcgt gttcaaccag gagggcctga
601acgccatcca gccaccctGC GTGGTCCAGA ATTTCATCAA CCACAACGCC GTCCTGTACA
661AGGTGTTCGT GGTTGGCGAG TCCTACACCG TGGTCCAGAG GCCCTCACTC AAGAACTTCT
721CCGCAGGCAC ATCAGACCGT GAGTCCATCT TCTTCAACAG CCACAACGTG TCAAAGCCGG
781AGTCGTCATC GGTCCTGACG GAGCTGGACA AGATCGAGGG CGTGTTCGAG CGGCCGAGCG
841ACGAGGTCAT CCGGGAGCTC TCCCGGGCCC TGCGGCAGGC ACTGGGCGTG TCACTCTTCG
901GCATCGACAT CATCATCAAC AACCAGACAG GGCAGCACGC CGTCATTGAC ATCAATGCCT
961TCCCAGGGGA CTGCCAAGTG TGCTTTATAG AAGGCTGGAA GACCGACTGC CCAACTTTCT
1021TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1081CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1141GTGGAAAGGA CGAGCCCATG ACCTAGTATA CAAAAAAACG CGTTAAGTCg acaatcaacc
1201tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)