Construct: ORF TRCN0000469387
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000406.1_s317c1
- Derived from:
- ccsbBroadEn_00888
- DNA Barcode:
- GCCCATGACCTAGTATACAAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITPK1 (3705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469387
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001142594.3 | 100% | 100% | |
| 2 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001142593.3 | 73.9% | 72.6% | (many diffs) |
| 3 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_014216.6 | 73.9% | 72.6% | (many diffs) |
| 4 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021262.1 | 60.2% | 56.5% | (many diffs) |
| 5 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | NM_001363707.1 | 45.3% | 44.2% | (many diffs) |
| 6 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021263.1 | 42.7% | 41.6% | (many diffs) |
| 7 | human | 3705 | ITPK1 | inositol-tetrakisphosphate ... | XM_017021264.1 | 42.7% | 41.6% | (many diffs) |
| 8 | mouse | 217837 | Itpk1 | inositol 1,3,4-triphosphate... | NM_172584.3 | 65.3% | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gacctttctg aaagggaaga gagttggcta ctggctgagc gagaagaaaa 121 tcaagaagct gaatttccag gccttcgccg agctgtgcag gaagcgaggg atggaggttg 181 tgcagctgaa ccttagccgg ccgatcgagg agcagggccc cctggacgtc atcatccaca 241 agctgactga cgtcatcctt gaagccgacc agaatgatag ccagtccctg gagctggtgc 301 acaggttcca ggagtacatc gatgcccacc ctgagaccat cgtcctggac ccgctccctg 361 ccatcagaac cctgcttgac cgctccaagt cctatgagct catccggaag attgaggcct 421 acatggaaga cgacaggatc tgctcgccac ccttcatgga gctcacgagc ctgtgcgggg 481 atgacaccat gcggctgctg gagaagaacg gcttgacttt cccattcatt tgcaaaacca 541 gagtggctca tggcaccaac tctcacgaga tggctatcgt gttcaaccag gagggcctga 601 acgccatcca gccaccctGC GTGGTCCAGA ATTTCATCAA CCACAACGCC GTCCTGTACA 661 AGGTGTTCGT GGTTGGCGAG TCCTACACCG TGGTCCAGAG GCCCTCACTC AAGAACTTCT 721 CCGCAGGCAC ATCAGACCGT GAGTCCATCT TCTTCAACAG CCACAACGTG TCAAAGCCGG 781 AGTCGTCATC GGTCCTGACG GAGCTGGACA AGATCGAGGG CGTGTTCGAG CGGCCGAGCG 841 ACGAGGTCAT CCGGGAGCTC TCCCGGGCCC TGCGGCAGGC ACTGGGCGTG TCACTCTTCG 901 GCATCGACAT CATCATCAAC AACCAGACAG GGCAGCACGC CGTCATTGAC ATCAATGCCT 961 TCCCAGGGGA CTGCCAAGTG TGCTTTATAG AAGGCTGGAA GACCGACTGC CCAACTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGAGCCCATG ACCTAGTATA CAAAAAAACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt