Construct: ORF TRCN0000469406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018091.1_s317c1
- Derived from:
- ccsbBroadEn_08466
- DNA Barcode:
- AACATACTCTTTGTTGAGCAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RADX (55086)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55086 | RADX | RPA1 related single strande... | NM_018015.6 | 99.9% | 99.8% | 97G>T;660C>A |
| 2 | human | 55086 | RADX | RPA1 related single strande... | NM_001184782.1 | 88.5% | 88.5% | 97G>T;660C>A;1442_1443ins291 |
| 3 | human | 55086 | RADX | RPA1 related single strande... | XR_001755707.2 | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2631
- ORF length:
- 2565
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggtgagtca ggacagcctg aggctggtcc ctcacatgca gggctagatt 121 ggccgaaccc tgagaggaat cgggctgggg tcccgggagg gttgatccga agagctggtt 181 cccaagggcc caggtcctgg atccaaaagg ttcttgagca gattatggac tcacctcgcc 241 agtgtgtcac cccctcggag gtggtgcctg taactgtgct ggccgtccag aggtacctgt 301 tagaggatga gccacgcgac acggtgccca agcctcccct ttattgctat gatgtgacga 361 tctcagatgg ggtgtaccag gagaagtgct acctggaccc cagcttgaac tctctcgtat 421 atcaaaatat tcttaaagtt ggcattcaaa tgagaatttc cagggtctca tgtctttaca 481 atgagaaaag gataggccag gggatcctgt gcatagataa cgtccactgt ggggagactt 541 cagacagtat ttctttagaa actcccttca gaaatagagc gcaccaggag aaaccagaga 601 ggcctttaag aggcgggaag agtcattacc tggcgctgtg gaataacgaa gatccctatg 661 gagatatctg gttaacagac aagcaacctg aggaacacaa ctttagcgat accaaaataa 721 tttcactttc tcatcttgaa atgacctgga ctaacagaag aaattttcct gcattgcttg 781 tgaggatctt acataaatca aaactgcgat actatggaaa acctgataaa aagatgattg 841 aaccatatca gacctttttg gaagttgctg acagttcagg cacagtgtca gtgattatgt 901 ggaatgccct gtgtcctgag tggtataaaa gtttgcgggt tggtttagtt cttctgcttc 961 aagactattc tgttaaaaag agttatccat tcagaataca gcctgtcccc gtggatccac 1021 agatcaaact aatttctaca atggaaatct gcctgaatct tcgagatccc ccaacaaata 1081 taattatcat tccagaaaag caggtgaaac cagaatggag actgccaaag ctaaatcacc 1141 gatttaccac aaggtcagaa ctggatgata tgccagaaaa ttgcatctgt gatgttattg 1201 gccttttagt ttttgtagga agggtccagc ggtcaaaaaa gaaagaaaac cgtgaagatt 1261 tttggtcata tcgctggatt cacattgctg acggtacttc agaacaacca tttatagtgg 1321 aactgttttc aacatcgcag ccagaaatct ttgaaaatat ttacccaatg gcatattttg 1381 tgtgtacaca gttgaaagtt gtcagaaatg acaatcaagt acctaagctg ctttacctca 1441 ccactacaaa tgagagtgga gtgtttatta ctggtcatag aggccagccg tatacgtatg 1501 atgccaaggt aaaaaacttt attcaatgga ttagaacaaa gtctgattcc ggggaacaga 1561 agaatatggt tattggtgga tattacccct atccaccagt gccagagaca ttttccaagt 1621 atagtagttc tattaaagtt gagtcgctct tgacagctat aagtgaagtc aggaaggaga 1681 ttgaagactt gcagtatagg gaacaaaagc gcattgcaat tcaggggata attactgcta 1741 taaagtatat cccccatagc agtgcgactg aaagtgcctc agcatcagaa acacttcgga 1801 atgctaacag accctcgacc tctcaagcag ctagagtaga aattcaagaa agaaatggta 1861 aacggcatca agatgatgag cctgtgaatt ctcagtattt ccaaactaca tctacaaatt 1921 taagtctgag caataaaata agaattcttc aaggcccaca cgctaatcca gttgctgtac 1981 ctcaaccagg agcttctgta caaacaaagg gaattaaacc gggcatgcca agcatcttca 2041 accgtcgagc aaatataaat gctaatctgc aagggaaagc cagaaaaact ataagtgata 2101 ggtgggagag tcagctgtgg agagagaaaa agtttggctt aatagatcac ctacactaca 2161 gccgtgttta tcctgaaagt attccacgga aatttatgtt tgaacacaga aagtttctta 2221 gtgaccagta taattctcag cctgcgaaaT ATGTACCACC AGAAGGAAGG CCCCCAAAAC 2281 TTGATGATTT TAAGAGCGCC CGAAGCCTTG GACATTTTGA AGTAACCATA CTAGGTCTGA 2341 ACCATGAGAT AGCAATCGAT GTTGCTTTCC TACCCATGTA TTGTCCAGAA GATATTCGAA 2401 CATCTCAAAT AGACACACTG TTGACCTCCA TGAATTACAG CTGTGCATAT CCACAGGACA 2461 CAACTGGAAA TGACCGATTG CCAGGTCCAA GAGCGGTTGC AGGTGATATT ATAAAAGCAG 2521 CAACTGAACT GGATAGAGTG CATATCGTCG GTATCTTGGA TATCTGTAAT TTGGGTAATA 2581 ATAAAGTGGA AGTCTATTTG CACAAGATTT ATAGTCCAGA GAATACTTCT TACCCAACTT 2641 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2701 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2761 CTTGTGGAAA GGACGAAACA TACTCTTTGT TGAGCAAAAA ACGCGTTAAG TCgacaatca 2821 acctctggat tacaaaattt gtgaaagatt