Construct: ORF TRCN0000469469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001564.1_s317c1
- Derived from:
- ccsbBroadEn_13824
- DNA Barcode:
- ACGGCCAAATCTGAAAACGATCGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAV3 (859)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 859 | CAV3 | caveolin 3 | NM_001234.5 | 99.5% | 99.3% | 99C>T;450delG |
| 2 | human | 859 | CAV3 | caveolin 3 | NM_033337.3 | 99.5% | 99.3% | 99C>T;450delG |
| 3 | mouse | 12391 | Cav3 | caveolin 3 | NM_007617.3 | 89.4% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggcagaagag cacacagatc tcgaggccca gatcgtcaag gatatccact 121 gcaaggagat tgacctggtg aaccgagacc ccaagaacat taatgaggac atagtcaagg 181 tggattttga agacgtgatc gcagagcctg tgggcaccTA CAGCTTTGAC GGCGTGTGGA 241 AGGTGAGCTA CACCACCTTC ACTGTCTCCA AGTACTGGTG CTACCGTCTG TTGTCCACGC 301 TGCTGGGCGT CCCACTGGCC CTGCTCTGGG GCTTCCTGTT CGCCTGCATC TCCTTCTGCC 361 ACATCTGGGC GGTGGTGCCA TGCATTAAGA GCTACCTGAT CGAGATCCAG TGCATCAGCC 421 ACATCTACTC ACTCTGCATC CGCACCTTCT GCAACCCACT CTTCGCGGCC CTGGGCCAGG 481 TCTGCAGCAG CATCAAGGTG GTGCTGCGGA AGGAGTCTAC CCAACTTTCT TGTACAAAGT 541 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 601 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 661 CGAACGGCCA AATCTGAAAA CGATCGCACG CGTTAAGTCg acaatcaacc tctggattac 721 aaaatttgtg aaagatt