Construct: ORF TRCN0000469487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017594.1_s317c1
- Derived from:
- ccsbBroadEn_08638
- DNA Barcode:
- CATTAGTCTGGCGCGATACGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf44 (56260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56260 | C8orf44 | chromosome 8 open reading f... | NR_161218.1 | 37.5% | (many diffs) | |
| 2 | human | 56260 | C8orf44 | chromosome 8 open reading f... | NR_161216.1 | 21.3% | 1_173del;616T>C;651_2230del | |
| 3 | human | 56260 | C8orf44 | chromosome 8 open reading f... | NR_161217.1 | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaagaatgag agttatctca accagccagc accccctatc cccatTCCCA 121 CACTTTCCCT CATGGGAGGC TGTCGGGAGC ACTTCGAAAA CCACTGGAAA GGCCGGGCAC 181 GGTGGCTCAT GCCTGTAATC CCAGCACTTT GGGAGGCCAA GGCAGGCAGA TCACCTGAGG 241 TCAGGAGTTC GAAACCAGCC TGGCCAACAT GGCGAAACCC CATCTTTACT AAAAATACGA 301 AAATTAGCCA GGTATTAGAA TTATTTCTGA ATTATCAGTC TCTCATTTGT GCTTTGGAGA 361 AGCAGAAAAG GCAAAAGGGG TCTTTGGCCA TCTTCTGCTG GAGCTTCCAG GGAGGATGTG 421 TCTCCAAGAG ACCAGATGTA CCGAGTTTGA AATCCCAGAA GCCCAAGAGG AAAAGAATCA 481 CAGGGAGGAA AAGACTGTCC AAAGGCTCCT GGAGTCTTCT GTTCTCTAAC CTTGGAAGGT 541 TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 661 GGCTTTATAT ATCTTGTGGA AAGGACGACA TTAGTCTGGC GCGATACGAC ATACGCGTTA 721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt