Construct: ORF TRCN0000469539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008742.1_s317c1
- Derived from:
- ccsbBroadEn_02000
- DNA Barcode:
- ACTGTAGCCCCAGCGCATATGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX4 (8723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8723 | SNX4 | sorting nexin 4 | NM_003794.4 | 100% | 100% | |
| 2 | human | 8723 | SNX4 | sorting nexin 4 | XM_017007414.2 | 93.3% | 93.3% | 787_882del |
| 3 | human | 8723 | SNX4 | sorting nexin 4 | NR_073435.2 | 48.8% | 1_30del;170_171ins122;1259_2390del | |
| 4 | mouse | 69150 | Snx4 | sorting nexin 4 | NM_080557.2 | 89% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaggcacct ccggaccccg agcggcagct ccagccggcg cccttggagc 121 cgctgggctc cccagacgct gggctggggg ctgcggtcgg caaggaagcg gagggggccg 181 gagaagagag ctctggggtc gacacgatga cacacaataa tttttggttg aagaagatag 241 aaatcagtgt ttcagaagca gaaaaacgaa ctggaagaaa tgccatgaac atgcaagaaa 301 catatactgc ttacctcatt gaaacaaggt cagttgaaca taccgatggt cagagtgtcc 361 taacagactc actatggcgg cgatatagtg aatttgagtt gttgagaagc taccttttag 421 tttactatcc acatattgtt gtgccacctc tgccagaaaa acgggcagaa tttgtttggc 481 ataaactctc tgctgacaac atggatccag attttgtgga gaggcgacgg attggtttag 541 aaaactttct cttgaggatt gcttcacatc ccatcctttg tagagacaaa atcttctatc 601 tgtttttaac acaggaaggt aactggaagg agactgtgaa tgaaactggg tttcagctga 661 aggcagactc caggttaaaa gcgcttaatg caacattcag agtgaaaaac ccagacaaga 721 gatttactga ccttaagcac tatagtgatg aactgcagtc tgtcatctca catcttcttc 781 gagtcagagc tagagtagca gatcgactct atggtgtata taaagtacat gggaattatg 841 gtcgagtttt cagtgaatgg agtgccatag aaaaagaaat gggtgatgga ctgcagagtg 901 ctggtcatca tatggatgtg tatgcatcTT CTATTGATGA TATTTTGGAA GATGAAGAAC 961 ATTATGCAGA TCAGTTAAAA GAGTATCTTT TTTATGCAGA AGCATTGCGG GCTGTGTGCA 1021 GGAAACATGA ACTTATGCAG TATGACTTGG AGATGGCTGC TCAGGACTTA GCATCCAAGA 1081 AGCAGCAGTG TGAGGAACTG GTAACTGGGA CTGTGAGAAC ATTCTCTTTG AAGGGAATGA 1141 CTACCAAGCT CTTTGGTCAA GAAACTCCAG AGCAGAGAGA AGCCAGAATA AAGGTGCTAG 1201 AAGAACAAAT AAATGAAGGA GAACAACAGC TAAAGTCTAA AAATCTGGAA GGCAGAGAAT 1261 TTGTGAAAAA CGCATGGGCT GATATTGAAC GCTTCAAAGA ACAAAAGAAC CGAGACTTAA 1321 AGGAGGCCCT CATAAGCTAT GCAGTCATGC AGATCAGTAT GTGCAAAAAG GGAATTCAAG 1381 TTTGGACCAA TGCTAAGGAA TGCTTTAGCA AGATGTACCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 AACTGTAGCC CCAGCGCATA TGCTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt