Construct: ORF TRCN0000469541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003953.1_s317c1
- Derived from:
- ccsbBroadEn_13762
- DNA Barcode:
- GGCTGGAGCAGCAGCGATTACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 53.3% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggtctcct ctcctcctca ctctcctcgc tcactgcaca gggtcctggg 121 cccagtctgt gctgacgcag ccgccctcag tgtctggggc cccagggcag agggtcacca 181 tctcctgcac tgggagcagc tccaacatcg gggcaggtta tgatgtacac tggtaccagc 241 agcttccagg aacagccccc aaactcctca tctatggtaa cagcaatcgg ccctcagggg 301 tccctgaccg attctctggc tccaagtctg gcaccTCAGC CTCCCTGGCC ATCACTGGGC 361 TCCAGGCTGA GGATGAGGCT GATTATTACT GCCAGTCCTA TGACAGCAGC CTGAGTGGTT 421 TTGTGGTATT CGGCGGAGGG ACCAAGCTGA CCGTCCTAGG TCAGCCCAAG GCTGCCCCCT 481 CGGTCACTCT GTTCCCGCCC TCCTCTGAGG AGCTTCAAGC CAACAAGGCC ACACTGGTGT 541 GTCTCATAAG TGACTTCTAC CCGGGAGCCG TGACAGTGGC CTGGAAGGCA GATAGCAGCC 601 CCGTCAAGGC GGGAGTGGAG ACCACCACAC CCTCCAAACA AAGCAACAAC AAGTACGCGG 661 CCAGCAGCTA TCTGAGCCTG ACGCCTGAGC AGTGGAAGTC CCACAGAAGC TACAGCTGCC 721 AGGTCACGCA TGAAGGGAGC ACCGTGGAGA AGACAGTGGC CCCTACAGAA TGTTCATACC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAGGCTGGAG CAGCAGCGAT TACAAAACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt