Construct: ORF TRCN0000469547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016067.1_s317c1
- Derived from:
- ccsbBroadEn_05333
- DNA Barcode:
- TTCTTTCTAGCAGAGTCTAGCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAOA (267012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 267012 | DAOA | D-amino acid oxidase activator | NM_172370.4 | 100% | 100% | |
| 2 | human | 267012 | DAOA | D-amino acid oxidase activator | XM_005254042.1 | 70.3% | 59.6% | (many diffs) |
| 3 | human | 267012 | DAOA | D-amino acid oxidase activator | NM_001161814.1 | 53.5% | 53.5% | 0_1ins213 |
| 4 | human | 267012 | DAOA | D-amino acid oxidase activator | NM_001161812.1 | 46.8% | 49.3% | 0_1ins193;89_197del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 528
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GCTGGAAAAG CTGATGGGTG CTGATTCTCT CCAGCTTTTC AGATCCAGAT 121 ATACATTGGG TAAAATCTAC TTCATAGGTT TTCAAAGGAG CATTCTTCTG AGCAAATCTG 181 AAAACTCTCT AAACTCTATT GCAAAGGAGA CAGAAGAAGG AAGAGAGACG GTAACAAGGA 241 AAGAAGGATG GAAGAGAAGG CATGAGGACG GCTATTTGGA AATGGCACAG AGGCATTTAC 301 AGAGATCATT ATGTCCTTGG GTCTCTTACC TTCCTCAGCC CTATGCAGAG CTTGAAGAAG 361 TAAGCAGCCA TGTTGGAAAA GTCTTCATGG CAAGAAACTA TGAGTTCCTT GCCTATGAGG 421 CCTCTAAGGA CCGCAGGCAG CCTCTAGAAC GAATGTGGAC CTGCAACTAC AACCAGCAAA 481 AAGACCAGTC ATGCAACCAC AAGGAAATAA CTTCTACCAA AGCTGAATTG CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGATTCTTTC TAGCAGAGTC TAGCTCAACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt