Construct: ORF TRCN0000469559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012178.1_s317c1
- Derived from:
- ccsbBroadEn_08089
- DNA Barcode:
- GGGGTGACTTGCCCTTAATCCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAT2B (27430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469559
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27430 | MAT2B | methionine adenosyltransfer... | NM_013283.5 | 99.9% | 99.7% | 905C>T |
| 2 | human | 27430 | MAT2B | methionine adenosyltransfer... | NM_182796.2 | 95.3% | 93.4% | (many diffs) |
| 3 | mouse | 108645 | Mat2b | methionine adenosyltransfer... | NM_134017.2 | 88.7% | 94% | (many diffs) |
| 4 | mouse | 108645 | Mat2b | methionine adenosyltransfer... | NM_001199274.1 | 84.7% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggggcgggag aaagagctct ctatacactt tgttcccggg agctgtcggc 121 tggtggagga ggaagttaac atccctaata ggagggttct ggttactggt gccactgggc 181 ttcttggcag agctgtacac aaagaatttc agcagaataa ttggcatgca gttggctgtg 241 gtttcagaag agcaagacca aaatttgaac aggttaatct gttggattct aatgcagttc 301 atcacatcat tcatgatttt cagccccatg ttatagtaca ttgtgcagca gagagaagac 361 cagatgttgt agaaaatcag ccagatgctg cctctcaact taatgtggat gcttctggga 421 atttagcaaa ggaagcagct gctgttggag catttctcat ctacattagc tcagattatg 481 tatttgatgg aacaaatcca ccttacagag aggaagacat accagctccc ctaaatttgt 541 atggcaaaac aaaattagat ggagaaaagg ctgtcctgga gaacaatcta ggagctgctg 601 ttttgaggat tcctattctg tatgGGGAAG TTGAAAAGCT CGAAGAAAGT GCTGTGACTG 661 TTATGTTTGA TAAAGTGCAG TTCAGCAACA AGTCAGCAAA CATGGATCAC TGGCAGCAGA 721 GGTTCCCCAC ACATGTCAAA GATGTGGCCA CTGTGTGCCG GCAGCTAGCA GAGAAGAGAA 781 TGCTGGATCC ATCAATTAAG GGAACCTTTC ACTGGTCTGG CAATGAACAG ATGACTAAGT 841 ATGAAATGGC ATGTGCAATT GCAGATGCCT TCAACCTCCC CAGCAGTCAC TTAAGACCTA 901 TTACTGACAG CCCTGTCCTA GGAGCACAAC GTCCGAGAAA TGCTCAGCTT GACTGCTCCA 961 AATTGGAGAT CTTGGGCATT GGCCAACGAA CACCATTTCG AATTGGAATC AAAGAATCAC 1021 TTTGGCCTTT CCTCATTGAC AAGAGATGGA GACAAACGGT CTTTCATTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGAGGGGTGA CTTGCCCTTA ATCCCTAACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt