Construct: ORF TRCN0000469566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011084.1_s317c1
- Derived from:
- ccsbBroadEn_03943
- DNA Barcode:
- TCGCGGAGCTTTAATCGAAGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GMCL1 (64395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | NM_178439.5 | 100% | 100% | |
| 2 | human | 64396 | GMCL2 | germ cell-less 2, spermatog... | NM_001358008.2 | 92.8% | 88.7% | (many diffs) |
| 3 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | XM_011533033.2 | 91% | 91% | 932_933ins138 |
| 4 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | XM_011533034.2 | 64% | 63.1% | (many diffs) |
| 5 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | XM_017004704.2 | 64% | 63.1% | (many diffs) |
| 6 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | XM_017004705.1 | 60.5% | 60.5% | 0_1ins609 |
| 7 | human | 64395 | GMCL1 | germ cell-less 1, spermatog... | XR_001738895.2 | 36.3% | 1_233del;1452_1554del;1882_4249del | |
| 8 | mouse | 23885 | Gmcl1 | germ cell-less, spermatogen... | NM_011818.3 | 85.6% | 88.3% | (many diffs) |
| 9 | mouse | 23885 | Gmcl1 | germ cell-less, spermatogen... | XM_006506056.2 | 46.8% | 49.2% | (many diffs) |
| 10 | mouse | 23885 | Gmcl1 | germ cell-less, spermatogen... | XR_377446.3 | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atcgttgagc agccgggtgc tgcgccagcc aagaccagcc cttgcccagc 121 aggcgcaggg tgccagggcg gggggctcgg cccggaggcc ggacactgga gacgatgcgg 181 cgggccacgg attctgttac tgtgcgggca gccacaagcg caagcggagc agcgggtcct 241 tctgctactg tcaccctgac tcggagacgg acgaggatga ggaggagggg gacgagcagc 301 agcggctcct caacacccct cgaaggaaaa aattaaagag tacatctaaa tatatttatc 361 aaacattatt tttgaatggt gaaaacagtg acattaagat ttgtgctcta ggagaagaat 421 ggagcttaca caaaatatat ttatgtcaat ctggctactt ttctagtatg ttcagtggtt 481 cttggaaaga atccagcatg aatattattg aactggagat tcctgaccag aacattgatg 541 tagaagcact gcaggttgca tttggttcac tgtatcgaga tgatgtcttg ataaagccca 601 gtcgagttgt tgccattttg gcagcagctt gtttgctgca gttggacggt ttaatacagc 661 agtgtggtga gacaatgaag gaaacagtta atgtgaaaac tgtatgtggc tattacacat 721 cagcagggac ctatggatta gattctgtaa agaaaaagtg ccttgaatgg cttctaaaca 781 atttgatgac tcaccagaat gttgaacttt ttaaagaact cagtataaat gtcatgaaac 841 agctcattgg ttcatctaac ttatttgtga tgcaagtgga gatggatata tacactgctc 901 taaaaaagtg gatgttcctt caacttgtgc cttcttggaa tggatcttta aaacagcttt 961 tgacagaaac agatgtctgg ttttctaaac agaggaaaga ttttgaaggt atggcctttc 1021 ttgaaactga acaaggaaaa ccatttgtgt cagtattcag acatttaagg ttacaatata 1081 ttatcagtga tctggcttct gcaagaatta ttgaacaaga tgctgtagta ccttcagaat 1141 ggctctcttc tgtgtataaa cagcagtggt ttgctatgct gcgggcagaa caggacagtg 1201 aggtggGGCC TCAAGAAATC AATAAAGAAG AACTAGAGGG AAACAGCATG AGGTGTGGTA 1261 GAAAGCTTGC CAAAGATGGT GAATACTGCT GGCGTTGGAC AGGTTTTAAC TTCGGCTTCG 1321 ACCTACTTGT AACTTACACC AATCGATACA TCATTTTCAA ACGCAATACA CTGAATCAGC 1381 CATGTAGCGG ATCTGTCAGT TTACAGCCTC GAAGGAGCAT AGCATTTAGA TTACGTTTGG 1441 CTTCTTTTGA TAGTAGTGGA AAACTAATAT GTAGTAGAAC AACTGGCTAT CAAATACTTA 1501 CACTTGAAAA GGATCAGGAA CAAGTGGTGA TGAACTTGGA CAGCAGGCTT CTGATCTTCC 1561 CTTTATATAT CTGCTGTAAC TTCTTGTATA TATCACCAGA AAAAAAGAAT TGTCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGATCGC GGAGCTTTAA TCGAAGTCAG ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt