Construct: ORF TRCN0000469581

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009449.1_s317c1
Derived from:
ccsbBroadEn_11693
DNA Barcode:
TGTGTAATGGACCCAGCGTGCCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP11B (23200)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469581
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23200 ATP11B ATPase phospholipid transpo... XM_017006008.1 11.8% 11.2% (many diffs)
2 human 23200 ATP11B ATPase phospholipid transpo... XM_011512597.2 10.6% 10.1% (many diffs)
3 human 23200 ATP11B ATPase phospholipid transpo... XM_005247243.2 10.2% 9.7% (many diffs)
4 human 23200 ATP11B ATPase phospholipid transpo... NM_014616.3 8.6% 8.2% (many diffs)
5 human 23200 ATP11B ATPase phospholipid transpo... XM_005247241.2 6.9% 4.8% 1_3174del;3317_3402del;3507_3508ins65
6 human 23200 ATP11B ATPase phospholipid transpo... XR_001740068.1 3.5% (many diffs)
7 human 23200 ATP11B ATPase phospholipid transpo... XR_001740067.1 3.4% (many diffs)
8 human 23200 ATP11B ATPase phospholipid transpo... XR_001740066.1 3.4% (many diffs)
9 mouse 76295 Atp11b ATPase, class VI, type 11B XM_006535568.2 5.8% 4.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
378
ORF length:
312
Sequence:
1TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGCATGTA TTTTGTGTTT ATTCAGCTCC TGTCAAGTGG TTCTGCTTGG TTTGCCATAA
121TCCTCATGGT TGTTACATGT CTATTTCTTG ATATCATAAA GAAGGTCTTT GACCGACACC
181TCCACCCTAC AAGTACTGAA AAGGCACAGC TTACTGAAAC AAATGCAGGT ATCAAGTGCT
241TGGACTCCAT GTGCTGTTTC CCGGAAGGAG AAGCAGCGTG TGCATCTGTT GGAAGAATGC
301TGGAACGAGT TATAGGAAGA TGTAGTCCAA CCCACATCAG CAGGTGTGAA ATCTCTCTAA
361GTAGCCTTTG CTGCAGATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
421TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
481GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTGTAA TGGACCCAGC
541GTGCCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)