Construct: ORF TRCN0000469584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009726.1_s317c1
- Derived from:
- ccsbBroadEn_11987
- DNA Barcode:
- CCTATTTTTCTCTCATCCACACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCMT1 (51451)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51451 | LCMT1 | leucine carboxyl methyltran... | XM_011545864.1 | 99.9% | 100% | 24G>C |
| 2 | human | 51451 | LCMT1 | leucine carboxyl methyltran... | NM_016309.3 | 89.2% | 80% | (many diffs) |
| 3 | human | 51451 | LCMT1 | leucine carboxyl methyltran... | XM_011545862.2 | 84.5% | 84.5% | 24G>C;472_473ins165 |
| 4 | human | 51451 | LCMT1 | leucine carboxyl methyltran... | NM_001032391.2 | 74% | 65.5% | (many diffs) |
| 5 | human | 51451 | LCMT1 | leucine carboxyl methyltran... | XM_005255354.4 | 65.5% | 65.5% | 0_1ins369 |
| 6 | mouse | 30949 | Lcmt1 | leucine carboxyl methyltran... | XM_006507917.3 | 43% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tccctgtgca agagaactcc catcagcagc gcccgacagc actctcctcc 121 tcactgccca acactgggtg gcaaatcttt tcatcttggc cactctgata ggctggacag 181 tgaggtcctt cttcctaggg cgcatggaaa cctgttgcag tcacctgggt ttgcggagtg 241 ggaggaggtt tgcagtaagc attggctact ggcatgaccc ttacatacag cactttgtga 301 gactgtctaa agagaggaaa gcccctgaaa tcaacagagg atattttgct cgagtccatg 361 gtgtcagtca gcttataaag gcatttctac ggaagacaga atgtcattgt caaattgtca 421 accttggggc aggcatggat accaccttct ggagattaaa ggatgaagat cttctcccaa 481 gtaaatattt tgaggttgac tttccaatga ttgtcacgag aaagctgcac agtatcaaat 541 gcaagcctcc cctatccagc cccattctag aactgcattc agaggacaca cttcagatgg 601 atggacacat actggattca aagagatatg ccgttattGG AGCAGATCTC CGAGACCTGT 661 CTGAACTGGA AGAGAAGCTA AAGAAATGTA ACATGAATAC ACAATTGCCA ACACTCCTGA 721 TAGCTGAATG TGTGCTGGTT TACATGACTC CAGAGCAGTC CGCAAACCTC CTGAAGTGGG 781 CAGCCAACAG TTTTGAGAGA GCCATGTTCA TAAACTACGA ACAGGTGAAC ATGGGTGATC 841 GGTTTGGGCA GATCATGATT GAAAACCTGC GGAGACGCCA GTGTGACCTG GCGGGAGTGG 901 AGACCTGCAA GTCATTAGAG TCACAGAAAG AACGGCTCCT GTCGAATGGG TGGGAAACAG 961 CATCGGCCGT CGACATGATG GAGTTGTACA ACAGGTTACC TCGAGCTGAA GTGAGCAGGA 1021 TAGAATCACT TGAATTCCTG GATGAAATGG AGCTGCTGGA GCAGCTCATG CGGCATTACT 1081 GCCTTTGCTG GGCAACCAAA GGAGGAAATG AGCTTGGGCT GAAGGAGATA ACTTATTACC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GACCTATTTT TCTCTCATCC ACACGTACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt