Construct: ORF TRCN0000469629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009368.1_s317c1
- Derived from:
- ccsbBroadEn_10888
- DNA Barcode:
- TTTGTAATGCTCGTGTTGCCATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB1 (3211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3211 | HOXB1 | homeobox B1 | NM_002144.4 | 70.5% | 65.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 774
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggactataat aggatgaact ccttcttaga gtacccactc tgtaaccggg 121 gacccagcgc ctacagcgcc cacagcgccc acagcgcccc aacctccttt cccccaagct 181 cggctcaggc ggttgacagc tatgcaagcg agggccgcta cggtgggggg ctgtccagcc 241 ctgcgtttca gcagaactcc ggctatcccg cccagcagcc gccttcgacc ctgggggtgc 301 ccttccccag ctctgcgccc tcggggtatg ctcctgccgc ctgcagcccc agctacgggc 361 cTTCTCAGTA CTACCCTCTG GGTCATTCAG AAGGAGACGG AGGCTATTTT CATCCCTCGA 421 GCTACGGGGC CCAGCTAGGG GGCTTGTCCG ATGGCTACGG AGCAGGTGGA GCCGGTCCGG 481 GGCCATATCC TCCGCAGCAT CCCCCTTATG GGAACGAGCA GACCGCAAGC TTTGCACCGG 541 CCTATGCTGA TCTCCTCTCC GAGGACAAGG AAACACCCTG CCCTTCAGAA CCTAACACCC 601 CCACGGCCCG GACCTTCGAC TGGATGAAGG TTAAGAGAAA CCCACCCAAG ACAGGTAGGG 661 CTCAGATGTG GCCACCCCTT CTCCGGGGCC CAAAGCATCT CTGCTTCCCC TGCAGTGACA 721 TGTCCTGGGT CTGGGCTGGG TTCTTCTCTT TCAGCGGCTC TGGTAGACAC AGGTTGCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAT TTGTAATGCT CGTGTTGCCA TAAACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att