Construct: ORF TRCN0000469652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018536.1_s317c1
- Derived from:
- ccsbBroadEn_14214
- DNA Barcode:
- TGGGCCTTTTCCTATCCCTTCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CMAS (55907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55907 | CMAS | cytidine monophosphate N-ac... | NM_018686.6 | 60.4% | 59.6% | 779delG;789_1302delinsG |
| 2 | human | 55907 | CMAS | cytidine monophosphate N-ac... | NR_135117.1 | 47.9% | 1_130del;909delG;920_1642del | |
| 3 | mouse | 12764 | Cmas | cytidine monophospho-N-acet... | NM_009908.2 | 53.2% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcggtggag aagggggccg ccacctccgt ctccaacccg cgggggcgac 121 cgtcccgggg ccggccgccg aagctgcagc gcaactctcg cggcggccag ggccgaggtg 181 tggagaagcc cccgcacctg gcagccctaa ttctggcccg gggaggcagc aaaggcatcc 241 ccctgaagaa cattaagcac ctggcggggg tcccgctcat tggctgggtc ctgcgtgcgg 301 ccctggattc aggggccttc cagagtgtat gggtttcgac agaccatgat gaaattgaga 361 atgtggccaa acaatttggt gcacaagttc atcgaagaag ttctgaagtt tcaaaagaca 421 gctctacctc actagatgcc atcatagaat ttcttaatta tcataatgag gttgacattg 481 taggaaatat tcaagctact tctccatgtt tacatcctac tgatcttcaa aaagttGCAG 541 AAATGATTCG AGAAGAAGGA TATGATTCTG TTTTCTCTGT TGTGAGACGC CATCAGTTTC 601 GATGGAGTGA AATTCAGAAA GGAGTTCGTG AAGTGACCGA ACCTCTGAAT TTAAATCCAG 661 CTAAACGGCC TCGTCGACAA GACTGGGATG GAGAATTATA TGAAAATGGC TCATTTTATT 721 TTGCTAAAAG ACATTTGATA GAGATGGGTT ACTTGCAGGG TGGAAAAATG GCATACTACG 781 AAATGCGAGC TGAACATAGT GTGGATATAG ATGTGGATAT TGATTGGCCT ATTGCAGAGC 841 AAAAGTATTA AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 901 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 961 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGGCCTTTTC CTATCCCTTC 1021 GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att