Construct: ORF TRCN0000469652

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018536.1_s317c1
Derived from:
ccsbBroadEn_14214
DNA Barcode:
TGGGCCTTTTCCTATCCCTTCGCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CMAS (55907)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469652
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55907 CMAS cytidine monophosphate N-ac... NM_018686.6 60.4% 59.6% 779delG;789_1302delinsG
2 human 55907 CMAS cytidine monophosphate N-ac... NR_135117.1 47.9% 1_130del;909delG;920_1642del
3 mouse 12764 Cmas cytidine monophospho-N-acet... NM_009908.2 53.2% 56.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
849
ORF length:
783
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ctcggtggag aagggggccg ccacctccgt ctccaacccg cgggggcgac
121cgtcccgggg ccggccgccg aagctgcagc gcaactctcg cggcggccag ggccgaggtg
181tggagaagcc cccgcacctg gcagccctaa ttctggcccg gggaggcagc aaaggcatcc
241ccctgaagaa cattaagcac ctggcggggg tcccgctcat tggctgggtc ctgcgtgcgg
301ccctggattc aggggccttc cagagtgtat gggtttcgac agaccatgat gaaattgaga
361atgtggccaa acaatttggt gcacaagttc atcgaagaag ttctgaagtt tcaaaagaca
421gctctacctc actagatgcc atcatagaat ttcttaatta tcataatgag gttgacattg
481taggaaatat tcaagctact tctccatgtt tacatcctac tgatcttcaa aaagttGCAG
541AAATGATTCG AGAAGAAGGA TATGATTCTG TTTTCTCTGT TGTGAGACGC CATCAGTTTC
601GATGGAGTGA AATTCAGAAA GGAGTTCGTG AAGTGACCGA ACCTCTGAAT TTAAATCCAG
661CTAAACGGCC TCGTCGACAA GACTGGGATG GAGAATTATA TGAAAATGGC TCATTTTATT
721TTGCTAAAAG ACATTTGATA GAGATGGGTT ACTTGCAGGG TGGAAAAATG GCATACTACG
781AAATGCGAGC TGAACATAGT GTGGATATAG ATGTGGATAT TGATTGGCCT ATTGCAGAGC
841AAAAGTATTA AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC
901TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT
961TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGGCCTTTTC CTATCCCTTC
1021GCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)