Construct: ORF TRCN0000469697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004249.1_s317c1
- Derived from:
- ccsbBroadEn_06340
- DNA Barcode:
- TAGCCACTGAGATGGGGTCGACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2H3 (2967)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2967 | GTF2H3 | general transcription facto... | NM_001516.5 | 99.8% | 100% | 738A>G |
| 2 | human | 2967 | GTF2H3 | general transcription facto... | NM_001271867.2 | 86.5% | 86.6% | 0_1ins123;615A>G |
| 3 | human | 2967 | GTF2H3 | general transcription facto... | NM_001271866.2 | 85.9% | 86% | 485_486ins129;609A>G |
| 4 | human | 2967 | GTF2H3 | general transcription facto... | NM_001271868.1 | 52.4% | 52.5% | 0_1ins438;300A>G |
| 5 | human | 2967 | GTF2H3 | general transcription facto... | XM_017019228.2 | 52.4% | 52.5% | 0_1ins438;300A>G |
| 6 | human | 2967 | GTF2H3 | general transcription facto... | XR_001748675.1 | 27% | (many diffs) | |
| 7 | mouse | 209357 | Gtf2h3 | general transcription facto... | NM_181410.3 | 84.6% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttcagacgaa gatgaattga atcttctggt tattgtagtt gatgccaacc 121 caatttggtg gggaaagcaa gcattaaagg aatctcagtt cactttatcc aaatgcatag 181 atgccgtgat ggtgctggga aattcgcatt tattcatgaa tcgttccaac aaacttgctg 241 tgatagcaag tcacattcaa gaaagccgat tcttatatcc tggaaagaat ggcagacttg 301 gagacttctt cggagaccct ggcaaccctc ctgaatttaa tccctctggg agtaaagatg 361 gaaaatacga acttttaacc tcagcaaatg aagttattgt tgaagagatt aaagatctaa 421 tgaccaaaag tgacataaag ggtcaacata cagaaacttt gctggcagga tccctggcca 481 aagccctttg ctacattcat agaatgaaCA AGGAAGTTAA AGACAATCAG GAAATGAAAT 541 CAAGGATATT GGTGATTAAG GCTGCAGAAG ACAGTGCGTT GCAGTATATG AACTTCATGA 601 ATGTCATCTT TGCAGCACAG AAACAGAATA TTTTGATTGA TGCCTGTGTT TTAGACTCCG 661 ACTCAGGGCT CCTCCAACAG GCTTGTGACA TCACGGGAGG ACTGTACCTG AAGGTGCCTC 721 AGATGCCTTC TCTTCTGCAG TATTTGCTGT GGGTGTTTCT TCCCGATCAA GATCAGAGAT 781 CTCAGTTAAT CCTCCCACCC CCGGTTCATG TTGACTACAG GGCTGCTTGC TTCTGTCATC 841 GAAATCTCAT TGAAATTGGT TATGTCTGTT CTGTGTGTTT GTCAATATTC TGCAATTTCA 901 GCCCCATTTG TACTACGTGC GAGACAGCCT TTAAAATTTC TCTGCCTCCA GTGCTGAAAG 961 CCAAGAAAAA GAAACTGAAA GTGTCTGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1021 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1081 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAGCC 1141 ACTGAGATGG GGTCGACACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1201 tgaaagatt