Construct: ORF TRCN0000469724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000619.1_s317c1
- Derived from:
- ccsbBroadEn_01771
- DNA Barcode:
- ATTATATTTCTCTTCTACCGTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VIM (7431)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469724
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7431 | VIM | vimentin | NM_003380.5 | 100% | 100% | |
| 2 | human | 7431 | VIM | vimentin | XM_006717500.2 | 100% | 100% | |
| 3 | mouse | 22352 | Vim | vimentin | NM_011701.4 | 91.7% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caccaggtcc gtgtcctcgt cctcctaccg caggatgttc ggcggcccgg 121 gcaccgcgag ccggccgagc tccagccgga gctacgtgac tacgtccacc cgcacctaca 181 gcctgggcag cgcgctgcgc cccagcacca gccgcagcct ctacgcctcg tccccgggcg 241 gcgtgtatgc cacgcgctcc tctgccgtgc gcctgcggag cagcgtgccc ggggtgcggc 301 tcctgcagga ctcggtggac ttctcgctgg ccgacgccat caacaccgag ttcaagaaca 361 cccgcaccaa cgagaaggtg gagctgcagg agctgaatga ccgcttcgcc aactacatcg 421 acaaggtgcg cttcctggag cagcagaata agatcctgct ggccgagctc gagcagctca 481 agggccaagg caagtcgcgc ctgggggacc tctacgagga ggagatgcgg gagctgcgcc 541 ggcaggtgga ccagctaacc aacgacaaag cccgcgtcga ggtggagcgc gacaacctgg 601 ccgaggacat catgcgcctc cgggagaaat tgcaggagga gatgcttcag agagaggaag 661 ccgaaaacac cctgcaatct ttcagacagg atgttgacaa tgcgtctctg gcacgtcttg 721 accttgaacg caaagtggaa tctttgcaag aagagattgc ctttttgaag aaactccacg 781 aagaggaaat ccaggagctg caggctcaga ttcaggaaca gcatgtccaa atcgatgtgg 841 atgtttccaa gcctgacctc acggctgccc tgcgtgacgt acgtcagcaa tatgaaagtg 901 tggctgccaa gaacctgcag gaggcagaag aatggtacaa atccaagttt gctgacctct 961 ctgaggctgc caaccggaac aatgacgccc TGCGCCAGGC AAAGCAGGAG TCCACTGAGT 1021 ACCGGAGACA GGTGCAGTCC CTCACCTGTG AAGTGGATGC CCTTAAAGGA ACCAATGAGT 1081 CCCTGGAACG CCAGATGCGT GAAATGGAAG AGAACTTTGC CGTTGAAGCT GCTAACTACC 1141 AAGACACTAT TGGCCGCCTG CAGGATGAGA TTCAGAATAT GAAGGAGGAA ATGGCTCGTC 1201 ACCTTCGTGA ATACCAAGAC CTGCTCAATG TTAAGATGGC CCTTGACATT GAGATTGCCA 1261 CCTACAGGAA GCTGCTGGAA GGCGAGGAGA GCAGGATTTC TCTGCCTCTT CCAAACTTTT 1321 CCTCCCTGAA CCTGAGGGAA ACTAATCTGG ATTCACTCCC TCTGGTTGAT ACCCACTCAA 1381 AAAGGACACT TCTGATTAAG ACGGTTGAAA CTAGAGATGG ACAGGTTATC AACGAAACTT 1441 CTCAGCATCA CGATGACCTT GAATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTATATTTCT 1621 CTTCTACCGT GAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att