Construct: ORF TRCN0000469751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016636.3_s317c1
- Derived from:
- ccsbBroadEn_05952
- DNA Barcode:
- TTCGCACGGAGGCTTACACGCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD2 (914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 914 | CD2 | CD2 molecule | NM_001767.5 | 99.8% | 99.4% | 798C>A;1039T>C |
| 2 | human | 914 | CD2 | CD2 molecule | NM_001328609.2 | 92.9% | 92.5% | 611_688del;876C>A;1117T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctttccatgt aaatttgtag ccagcttcct tctgattttc aatgtttctt 121 ccaaaggtgc agtctccaaa gagattacga atgccttgga aacctggggt gccttgggtc 181 aggacatcaa cttggacatt cctagttttc aaatgagtga tgatattgac gatataaaat 241 gggaaaaaac ttcagacaag aaaaagattg cacaattcag aaaagagaaa gagactttca 301 aggaaaaaga tacatataag ctatttaaaa atggaactct gaaaattaag catctgaaga 361 ccgatgatca ggatatctac aaggtatcaa tatatgatac aaaaggaaaa aatgtgttgg 421 aaaaaatatt tgatttgaag attcaagaga gggtctcaaa accaaagatc tcctggactt 481 gtatcaacac aaccctgacc tgtgaggtaa tgaatggaac tgaccccgaa ttaaacctgt 541 atcaagatgg gaaacatcta aaactttctc agagggtcat cacacacaag tggaccacca 601 gcctgagtgc aaaattcaag tgcacagcag ggaacaaagt cagcaaggaa tccagtgtcg 661 agcctgtcag ctgtccagag aaaggtctgg acatctatct catcattggc atatgtggag 721 gaggcagcct cttgatggtc tttgtggcac tgctcgtttt ctatatcacc aaaaggaaaa 781 aacagaggag tcggagaaat gatgaggagc tggagacaag agcccacaga gtagctactG 841 AAGAAAGGGG CCGGAAGCCC CAACAAATTC CAGCTTCAAC CCCTCAGAAT CCAGCAACTT 901 CCCAACATCC TCCTCCACCA CCTGGTCATC GTTCCCAGGC ACCTAGTCAT CGTCCCCCGC 961 CTCCTGGACA CCGTGTTCAG CACCAGCCTC AGAAGAGGCC TCCTGCTCCG TCGGGCACAC 1021 AAGTTCACCA GCAGAAAGGC CCGCCCCTCC CCAGACCTCG AGTTCAGCCA AAACCTCCCC 1081 ATGGGGCAGC AGAAAACTCA TTGCCCCCTT CCTCTAATTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGATTCGCA CGGAGGCTTA CACGCTAGAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt