Construct: ORF TRCN0000469809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009559.1_s317c1
- Derived from:
- ccsbBroadEn_15437
- DNA Barcode:
- GGTTCATCTCATCGGCATGGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2F1 (2962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2962 | GTF2F1 | general transcription facto... | NM_002096.3 | 99.8% | 99.8% | 813G>A;1372G>A |
| 2 | human | 2962 | GTF2F1 | general transcription facto... | XM_024451475.1 | 95.2% | 95.2% | 813G>A;898_972del;1447G>A |
| 3 | mouse | 98053 | Gtf2f1 | general transcription facto... | NM_133801.2 | 84.5% | 91.2% | (many diffs) |
| 4 | mouse | 98053 | Gtf2f1 | general transcription facto... | XM_006525194.3 | 79.9% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1617
- ORF length:
- 1551
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccctaggc cctagcagcc agaatgtcac tgaatacgtc gttcgagttc 121 ctaagaatac aaccaaaaaa tataacatca tggcttttaa tgcagccgac aaagtcaact 181 ttgctacgtg gaatcaggct cggctggagc gggacttgag caacaagaaa atctaccaag 241 aggaggagat gcccgaatcg ggcgcgggca gtgagttcaa ccgcaagctt cgggaggagg 301 ctcggaggaa gaagtacggc atcgtcctca aggagttccg gcccgaggac cagccctggc 361 tgctccgggt caacggcaaa tcaggcagga agttcaaggg catcaagaag ggaggcgtaa 421 cagagaacac gtcctactac atcttcaccc agtgccccga cggggccttc gaggccttcc 481 ccgtgcacaa ctggtacaat ttcacaccgc tggcccggca tcgcacgctc actgccgagg 541 aggccgagga ggagtgggag aggaggaaca aggtgctgaa ccacttcagc atcatgcagc 601 agcggcggct caaggatcag gaccaggacg aggatgagga ggagaaggag aaacgtggcc 661 gcaggaaggc gagcgagctg cgcatccacg acctggagga cgacctggag atgtcgtccg 721 atgccagtga tgccagtggt gaggaggggg gcagagtccc caaggccaag aagaaggcgc 781 cgctggccaa gggcggcagg aaaaagaaga agaagaaggg ttcagacgac gaggccttcg 841 aggacagcga tgatggggac ttcgagggcc aagaggtaga ctacatgtca gacggctcca 901 gtagctccca agaagagcct gagagcaagg ccaaggcgcc gcagcaggag gaggggccca 961 agggtgtcga tgagcagagc gacagtagtg aggagagtga ggaggagaag ccgcctgagg 1021 aggacaagga ggaggaggag gagaagaagg cacccacccc gcaggagaag aagcgcagga 1081 aagacagcag cgaggagtcg gacagctcag aggagagcga cattgacagc gaggcctcct 1141 cagccctctt catggcgaag aagaagacgc cacCCAAGAG AGAGCGGAAG CCGTCGGGAG 1201 GGAGCTCAAG GGGCAACAGC CGCCCAGGCA CGCCCAGCGC AGAGGGTGGC AGCACCTCCT 1261 CCACCCTGCG GGCGGCTGCC AGCAAACTCG AGCAAGGGAA GCGGGTGAGC GAGATGCCTG 1321 CAGCCAAGCG GTTGCGGCTG GACACGGGAC CCCAGAGCCT GTCTGGGAAG TCGACACCCC 1381 AGCCACCATC AGGCAAGACA ACACCCAACA GCGGCGACGT GCAGGTGACT GAGGATACCG 1441 TGCGCCGCTA CCTGACACGG AAGCCCATGA CCACTAAGGA CCTGCTGAAA AAGTTCCAGA 1501 CCAAGAAGAC AGGGCTGAGC AGCGAGCAGA CAGTGAACGT GTTGGCCCAG ATCCTCAAGC 1561 GACTCAACCC CGAGCGCAAG ATGATCAACG ACAAAATGCA CTTCTCCCTC AAGGAGTGCC 1621 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1681 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1741 ATATATCTTG TGGAAAGGAC GAGGTTCATC TCATCGGCAT GGTCCAACGC GTTAAGTCga 1801 caatcaacct ctggattaca aaatttgtga aagatt