Construct: ORF TRCN0000469835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001843.1_s317c1
- Derived from:
- ccsbBroadEn_10743
- DNA Barcode:
- AAATCTCAATTGCCCCACTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COMT (1312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469835
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1312 | COMT | catechol-O-methyltransferase | NM_007310.3 | 82.2% | 81.9% | 1_117del;322G>A |
| 2 | human | 1312 | COMT | catechol-O-methyltransferase | NM_000754.4 | 67% | 66.7% | 1_267del;472G>A |
| 3 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135161.2 | 67% | 66.7% | 1_267del;472G>A |
| 4 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135162.2 | 67% | 66.7% | 1_267del;472G>A |
| 5 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001362828.2 | 67% | 66.7% | 1_267del;472G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgtgggcgac aagaaaggca agatcgtgga cgccgtgatt caggagcacc 121 agccctccgt gctgctggag ctgggggcct actgtggcta ctcagctgtg cgcatggccc 181 gccTGCTGTC ACCAGGGGCG AGGCTCATCA CCATCGAGAT CAACCCCGAC TGTGCCGCCA 241 TCACCCAGCG GATGGTGGAT TTCGCTGGCA TGAAGGACAA GGTCACCCTT GTGGTTGGAG 301 CGTCCCAGGA CATCATCCCC CAGCTGAAGA AGAAGTATGA TGTGGACACA CTGGACATGG 361 TCTTCCTCGA CCACTGGAAG GACCGGTACC TGCCGGACAC GCTTCTCTTG GAGGAATGTG 421 GCCTGCTGCG GAAGGGGACA GTGCTACTGG CTGACAACGT GATCTGCCCA GGTGCGCCAG 481 ACTTCCTAGC ACACGTGCGC GGGAGCAGCT GCTTTGAGTG CACACACTAC CAATCGTTCC 541 TGGAATACAG GGAGGTGGTG GACGGCCTGG AGAAGGCCAT CTACAAGGGC CCAGGCAGCG 601 AAGCAGGGCC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA TCTCAATTGC CCCACTTTTA 781 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t