Construct: ORF TRCN0000469844

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013769.1_s317c1
Derived from:
ccsbBroadEn_02705
DNA Barcode:
CCAAGAACCAGATTACGTAGCTAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SIRT2 (22933)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469844
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22933 SIRT2 sirtuin 2 NM_030593.3 100% 100%
2 human 22933 SIRT2 sirtuin 2 XM_006723111.1 100% 100%
3 human 22933 SIRT2 sirtuin 2 XM_011526654.2 100% 100%
4 human 22933 SIRT2 sirtuin 2 NM_012237.4 90.4% 90.4% 1_111del
5 human 22933 SIRT2 sirtuin 2 XM_011526655.1 89.9% 89.2% (many diffs)
6 human 22933 SIRT2 sirtuin 2 XM_017026500.1 72.6% 69.8% (many diffs)
7 human 22933 SIRT2 sirtuin 2 NM_001193286.1 66.4% 65% 681_682ins152;702_703ins202
8 human 22933 SIRT2 sirtuin 2 NR_034146.1 52.5% 1_336del;1393_2009del
9 human 22933 SIRT2 sirtuin 2 XR_001753639.2 45.6% 1_190del;953_1423del;1718_2315del
10 human 22933 SIRT2 sirtuin 2 XR_001753638.2 44.7% 1_236del;999_1469del;1764_2361del
11 mouse 64383 Sirt2 sirtuin 2 NM_001122765.1 85.4% 87.5% (many diffs)
12 mouse 64383 Sirt2 sirtuin 2 NM_022432.4 77.3% 79.1% (many diffs)
13 mouse 64383 Sirt2 sirtuin 2 NM_001122766.1 77.3% 77.5% (many diffs)
14 mouse 64383 Sirt2 sirtuin 2 XM_017312216.1 65.1% 66.4% (many diffs)
15 mouse 64383 Sirt2 sirtuin 2 XM_017312215.1 62% 59.8% (many diffs)
16 mouse 64383 Sirt2 sirtuin 2 XM_017312214.1 58.2% 55.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1122
ORF length:
1056
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cttcctgcgg aacttattct cccagacgct cagcctgggc agccagaagg
121agcgtctgct ggacgagctg accttggaag gggtggcccg gtacatgcag agcgaacgct
181gtcgcagagt catctgtttg gtgggagctg gaatctccac atccgcaggc atccccgact
241ttcgctctcc atccaccggc ctctatgaca acctagagaa gtaccatctt ccctacccag
301aggccatctt tgagatcagc tatttcaaga aacatccgga acccttcttc gccctcgcca
361aggaactcta tcctgggcag ttcaagccaa ccatctgtca ctacttcatg cgcctgctga
421aggacaaggg gctactcctg cgctgctaca cgcagaacat agataccctg gagcgaatag
481ccgggctgga acaggaggac ttggtggagg cgcacggcac cttctacaca tcacactgcg
541tcagcgccag ctgccggcac gaatacccgc taagctggat gaaagagaag atcttctctg
601aggtgacgcc caagtgtgaa gactgtcaga gcctggtgaa gcctgatatc gtcttttttg
661gtgagagcct cccagcgcgt ttcttctcct gtatgcagtc agacttcctg aaggtggacc
721tcctcctggt catgggtacc tccttgcagg tgcagccctt tgcctcccTC ATCAGCAAGG
781CACCCCTCTC CACCCCTCGC CTGCTCATCA ACAAGGAGAA AGCTGGCCAG TCGGACCCTT
841TCCTGGGGAT GATTATGGGC CTCGGAGGAG GCATGGACTT TGACTCCAAG AAGGCCTACA
901GGGACGTGGC CTGGCTGGGT GAATGCGACC AGGGCTGCCT GGCCCTTGCT GAGCTCCTTG
961GATGGAAGAA GGAGCTGGAG GACCTTGTCC GGAGGGAGCA CGCCAGCATA GATGCCCAGT
1021CGGGGGCGGG GGTCCCCAAC CCCAGCACTT CAGCTTCCCC CAAGAAGTCC CCGCCACCTG
1081CCAAGGACGA GGCCAGGACA ACAGAGAGGG AGAAACCCCA GTGCCCAACT TTCTTGTACA
1141AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1201AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1261AGGACGACCA AGAACCAGAT TACGTAGCTA AACGCGTTAA GTCgacaatc aacctctgga
1321ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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