Construct: ORF TRCN0000469856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002722.1_s317c1
- Derived from:
- ccsbBroadEn_02169
- DNA Barcode:
- ATACTATTTCAACTCCACATACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMER1 (9456)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_004272.5 | 100% | 100% | |
| 2 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277077.1 | 63.2% | 63.2% | 294_295ins390 |
| 3 | human | 9456 | HOMER1 | homer scaffold protein 1 | XM_017010059.2 | 50.7% | 49.5% | (many diffs) |
| 4 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277078.1 | 49.8% | 49.4% | 528_529delGAinsTTC;531_532ins530 |
| 5 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001284189.2 | 91.1% | 98.5% | (many diffs) |
| 6 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_147176.4 | 88.1% | 95.3% | (many diffs) |
| 7 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_152134.3 | 87% | 93.7% | (many diffs) |
| 8 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001347598.1 | 50.3% | 50.4% | (many diffs) |
| 9 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_011982.4 | 47.8% | 49.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaacaacct atcttcagca ctcgagctca tgtcttccaa attgacccaa 121 acacaaagaa gaactgggta cccaccagca agcatgcagt tactgtgtct tatttctatg 181 acagcacaag aaatgtgtat aggataatca gtttagatgg ctcaaaggca ataataaata 241 gtaccatcac cccaaacatg acatttacta aaacatctca gaagtttggc cagtgggctg 301 atagccgggc aaacaccgtt tatggattgg gattctcctc tgagcatcat ctttcgaaat 361 ttgcagaaaa gtttcaggaa tttaaagaag ctgctcgact agcaaaggaa aaatcacaag 421 agaagatgga acttaccagt acaccttcac aggaatccgc aggcggggat cttcagtctc 481 ctttaacacc ggaaagtatc aacgggacag atgatgaaag aacacctgat gtgacacaga 541 actcagagcc aagggctgaa ccaactcaga atgcattgcc attttcacat agttcagcaa 601 tcagcaaaca ttgggaggct gaactggcta ccctcaaagg aaataatgcc aAACTCACTG 661 CAGCCCTGCT GGAGTCCACT GCCAATGTGA AACAATGGAA ACAGCAACTT GCTGCCTATC 721 AAGAGGAAGC AGAACGTCTG CACAAGCGGG TGACTGAACT TGAATGTGTT AGTAGCCAAG 781 CAAATGCAGT ACATACTCAT AAGACAGAAT TAAATCAGAC AATACAAGAA CTGGAAGAGA 841 CACTGAAACT GAAGGAAGAG GAAATAGAAA GGTTAAAACA AGAAATTGAT AATGCCAGAG 901 AACTACAAGA ACAGAGGGAT TCTTTGACTC AGAAACTACA GGAAGTAGAA ATTCGGAACA 961 AAGACCTGGA GGGACAACTG TCTGACTTAG AGCAACGTCT GGAGAAAAGT CAGAATGAAC 1021 AAGAAGCTTT TCGCAATAAC CTGAAGACAC TCTTAGAAAT TCTGGATGGA AAGATATTTG 1081 AACTAACAGA ATTACGAGAT AACTTGGCCA AGCTACTAGA ATGCAGCTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAATACTAT TTCAACTCCA CATACCGACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt