Construct: ORF TRCN0000469905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011493.1_s317c1
- Derived from:
- ccsbBroadEn_14182
- DNA Barcode:
- CTACCGATAGCCGTCAAGCCGAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLN6 (54982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54982 | CLN6 | CLN6 transmembrane ER protein | NM_017882.3 | 99.6% | 17.3% | 155C>T;167G>A;187delC |
| 2 | mouse | 76524 | Cln6 | ceroid-lipofuscinosis, neur... | NM_001033175.2 | 86% | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 231
- ORF length:
- 165
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgacgcgg aggcggcagc acctgggagc gacgggcggc ccaggcgcgc 121 agctgggcgc ctccttcctg caggccaggc atggctctgt gagcgctgat gaggctgccc 181 gcacggctcc cttccacctc gacctctggt tctacttcat actgcagaac taggttctgg 241 actttgggcg tccattgcca tgctggtatt ccctctcgag tggtttccac tcaacaagcc 301 cagtgttggg gactacttcc acatggccta caacgtcatc acgccctttc tcttgctcaa 361 gctcatcgag cggtcccccc gcaccctgcc acgctccatc acgtacgtga gcatcatcat 421 cttcatcatg ggtgccagca tccacctggt gggtgactct gtcaaccacc gcctgctctt 481 cagtggctac cagcaccacc tgtctgtccg tgagaacccc atcatcaaga atctcaagcc 541 ggagacgctg atcgactcct ttgagctgct ctactattat gatgagtacc tgggtcactg 601 catgtggtac atccccttcT TCCTCATCCT CTTCATGTAC TTCAGCGGCT GCTTTACTGC 661 CTCTAAAGCT GAGAGCTTGA TTCCAGGGCC TGCCCTGCTC CTGGTGGCAC CCAGTGGCCT 721 GTACTACTGG TACCTGGTCA CCGAGGGCCA GATCTTCATC CTCTTCATCT TCACCTTCTT 781 CGCCATGCTG GCCCTCGTCC TGCACCAGAA GCGCAAGCGC CTCTTCCTGG ACAGCAACGG 841 CCTCTTCCTC TTCTCCTCCT TCGCACTGAC CCTCTTGCTT GTGGCGCTCT GGGTCGCCTG 901 GCTGTGGAAT GACCCTGTTC TCAGGAAGAA GTACCCGGGT GTCATCTACG TCCCTGAGCC 961 CTGGGCTTTC TACACCCTTC ACGTCAGCAG TCGGCACTGC CCAACTTTCT TGTACAAAGT 1021 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1081 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1141 CGACTACCGA TAGCCGTCAA GCCGAACACG CGTTAAGTCg acaatcaacc tctggattac 1201 aaaatttgtg aaagatt