Construct: ORF TRCN0000469911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007070.1_s317c1
- Derived from:
- ccsbBroadEn_12320
- DNA Barcode:
- AATCATGCAATTTGTCCAGCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTWD1 (56986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_011521815.2 | 45.2% | 38.6% | (many diffs) |
| 2 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022425.1 | 45.2% | 38.6% | (many diffs) |
| 3 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022426.1 | 45.2% | 38.6% | (many diffs) |
| 4 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022423.2 | 38.5% | 33.2% | (many diffs) |
| 5 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022424.1 | 38.5% | 33.2% | (many diffs) |
| 6 | human | 56986 | DTWD1 | DTW domain containing 1 | NM_001144955.2 | 33.6% | 29.1% | (many diffs) |
| 7 | human | 56986 | DTWD1 | DTW domain containing 1 | NM_020234.6 | 33.6% | 29.1% | (many diffs) |
| 8 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022419.1 | 33.6% | 29.1% | (many diffs) |
| 9 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022420.1 | 33.6% | 29.1% | (many diffs) |
| 10 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022421.1 | 33.6% | 29.1% | (many diffs) |
| 11 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022422.1 | 33.6% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC TCTCAATCCA CCTATATTTC TCAAACGAAG TGAAGAAAAT AGTTCAAAAT 121 TTGTGGAAAC AAAACAGTCA CAAACTACTT CCATAGCTTC AGAAGATCCC CTTCAAAACT 181 TATGTTTAGC ATCTCAAGAA GTTCTTCAAA AAGCTCAGCA AAGTGGGAGA TCAAAATGTC 241 TCAAATGTGG TGGTTCCAGA ATGTTCTACT GCTATACATG TTATGTTCCA GTTGAAAATG 301 TACCTATTGA ACAGATTCCA CTTGTGAAGT TTAGCCTATA TCATCTTGGC CAGTCCATGG 361 TCTCCTCAGC ATCTAAAATC ACATGTATAG GCTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 541 TCATGCAATT TGTCCAGCTT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt