Construct: ORF TRCN0000469912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010833.1_s317c1
- Derived from:
- ccsbBroadEn_10146
- DNA Barcode:
- GACCCCGGGATCCTGGCCCTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJB3 (414061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469912
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414061 | DNAJB3 | DnaJ heat shock protein fam... | NM_001001394.4 | 99.7% | 100% | 216G>C |
| 2 | human | 100286922 | LOC100286922 | DnaJ heat shock protein fam... | NR_037694.1 | 38.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggactactac gaggtgctgg acgtgccccg gcaggccTCA TCCGAGGCCA 121 TCAAGAAGGC GTACCGCAAG CTGGCGCTCA AGTGGCACCC CGACAAAAAC CCTGAGAACA 181 AGGAGGAAGC GGAGAGGAGA TTCAAGCAGG TGGCCGAGGC CTACGAGGTG TTGTCGGACG 241 CCAAGAAACG CGATATCTAT GACCGCTATG GCGAGGCGGG CGCGGAGGGC GGCTGCACAG 301 GCGGCAGGCC CTTCGAGGAC CCCTTCGAGT ACGTCTTCAG CTTCCGCGAC CCAGCCGACG 361 TCTTCAGGGA GTTCTTCGGC GGCCAGGACC CATTCTCCTT TGACCTCTTG GGAAACCCGC 421 TGGAGAATAT TTTGGGGGGG TCAGAGGAAC TGCTGGGGAA GCAGAAGCAG AGCGTCTGCA 481 CCCCTTTTCT CTGCCTTCAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGACC CCGGGATCCT 661 GGCCCTTGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt