Construct: ORF TRCN0000469916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003404.1_s317c1
- Derived from:
- ccsbBroadEn_01256
- DNA Barcode:
- ATCCCTGTTCAGGCCCTCTGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1G (5496)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5496 | PPM1G | protein phosphatase, Mg2+/M... | NM_177983.3 | 100% | 100% | |
| 2 | mouse | 14208 | Ppm1g | protein phosphatase 1G (for... | NM_008014.3 | 91.5% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1704
- ORF length:
- 1638
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgcctacctc tcccagccca acacggtgaa gtgctccggg gacggggtcg 121 gcgccccgcg cctgccgctg ccctacggct tctccgccat gcaaggctgg cgcgtctcca 181 tggaggatgc tcacaactgt attcctgagc tggacagtga gacagccatg ttttctgtct 241 acgatggaca tggaggggag gaagttgcct tgtactgtgc caaatatctt cctgatatca 301 tcaaagatca gaaggcctac aaggaaggca agctacagaa ggctttagaa gatgccttct 361 tggctattga cgccaaattg accactgaag aagtcattaa agagctggca cagattgcag 421 ggcgacccac tgaggatgaa gatgaaaaag aaaaagtagc tgatgaagat gatgtggaca 481 atgaggaggc tgcactgctg catgaagagg ctaccatgac tattgaagag ctgctgacac 541 gctacgggca gaactgtcac aagggccctc cccacagcaa atctggaggt gggacaggcg 601 aggaaccagg gtcccagggc ctcaatgggg aggcaggacc tgaggactca actagggaaa 661 ctccttcaca agaaaatggc cccacagcca aggcctacac aggcttttcc tccaactcgg 721 aacgtgggac tgaggcaggc caagttggtg agcctggcat tcccactggt gaggctgggc 781 cttcctgctc ttcagcctct gacaagctgc ctcgagttgc taagtccaag ttctttgagg 841 acagtgagga tgagtcagat gaggcggagg aagaagagga agacagtgag gaatgcagcg 901 aggaagagga tggctacagc agtgaggagg cagagaatga ggaagatgag gatgacaccg 961 aggaggctga agaggacgat gaagaagaag aagaagagat gatggtgcca gggatggaag 1021 gcaaagagga gcctggctct gacagtggta caacagcggt ggtggccctg atacgaggga 1081 agcagttgat tgtagccaac gcaggagact ctcgctgtgt ggtatctgag gctggcaaag 1141 ctttagacat gtcctatgat cacaaaccag aggatgaagt agaactagca cgcatcaaga 1201 atgctggtgg caaggtcacc atggatgggc gagtcaacgg gggcctcaac ctctccagag 1261 ccattgggga ccacttctat aagagaaaca agaacctgcc acctgaggaa cagatgattt 1321 cagcccttcc tGACATCAAG GTGCTGACTC TCACTGACGA CCATGAATTC ATGGTCATTG 1381 CCTGTGATGG CATCTGGAAT GTGATGAGCA GCCAGGAAGT TGTAGATTTC ATTCAATCAA 1441 AGATCAGCCA GCGTGATGAA AATGGGGAGC TTCGGTTATT GTCATCCATT GTGGAAGAGC 1501 TGCTGGATCA GTGCCTGGCA CCAGACACTT CTGGGGATGG TACAGGGTGT GACAACATGA 1561 CCTGCATCAT CATTTGCTTC AAGCCCCGAA ACACAGCAGA GCTCCAGCCA GAGAGTGGCA 1621 AGCGAAAACT AGAGGAGGTG CTCTCTACTG AGGGGGCTGA AGAAAATGGC AACAGCGACA 1681 AGAAGAAGAA GGCCAAGCGA GACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1741 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1801 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCCCTGTTCA 1861 GGCCCTCTGT GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1921 att