Construct: ORF TRCN0000469935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002694.1_s317c1
- Derived from:
- ccsbBroadEn_02921
- DNA Barcode:
- CCCATGAGTAGGCCGAAGAGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERBP1 (26135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018067.2 | 100% | 100% | |
| 2 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018068.2 | 98.5% | 98.5% | 605_606insTTCTTTTTCACATTACAG |
| 3 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_001018069.2 | 96.3% | 96.3% | 695_696ins45 |
| 4 | human | 26135 | SERBP1 | SERPINE1 mRNA binding prote... | NM_015640.4 | 94.8% | 94.8% | 605_606insTTCTTTTTCACATTACAG;677_678ins45 |
| 5 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_025814.2 | 93.2% | 98.2% | (many diffs) |
| 6 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113564.1 | 91.7% | 96.8% | (many diffs) |
| 7 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113565.1 | 89.7% | 94.8% | (many diffs) |
| 8 | mouse | 66870 | Serbp1 | serpine1 mRNA binding prote... | NM_001113566.1 | 88.2% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgggcactta caggaaggct tcggctgcgt ggtcaccaac cgattcgacc 121 agttatttga cgacgaatcg gaccccttcg aggtgctgaa ggcagcagag aacaagaaaa 181 aagaagccgg cgggggcggc gttgggggcc ctggggccaa gagcgcagct caggccgcgg 241 cccagaccaa ctccaacgcg gcaggcaaac agctgcgcaa ggagtcccag aaagaccgca 301 agaacccgct gccccccagc gttggcgtgg ttgacaagaa agaggagacg cagccgcccg 361 tggcgcttaa gaaagaagga ataagacgag ttggaagaag acctgatcaa caacttcagg 421 gtgaagggaa aataattgat agaagaccag aaaggcgacc acctcgtgaa cgaagattcg 481 aaaagccact tgaagaaaag ggtgaaggag gcgaattttc agttgataga ccgattattg 541 accgacctat tcgaggtcgt ggtggtcttg gaagaggtcg agggggccgt ggacgtggaa 601 tgggccgagg agatggattt gattctcgtg gcaaacgtga atttgatagg catagtggaa 661 gtgatagatc ttctttttca cattacagtg gcctgaagca cgaggacaaa cgtggaggta 721 gcggatctca caactgggga actgtcaaag acgaattaac agagtccccc aaatacattc 781 agaaacaaat atcttataat tacagtgact tGGATCAATC AAATGTGACT GAGGAAACAC 841 CTGAAGGTGA AGAACATCAT CCAGTGGCAG ACACTGAAAA TAAGGAGAAT GAAGTTGAAG 901 AGGTAAAAGA GGAGGGTCCA AAAGAGATGA CTTTGGATGA GTGGAAGGCT ATTCAAAATA 961 AGGACCGGGC AAAAGTAGAA TTTAATATCC GAAAACCAAA TGAAGGTGCT GATGGGCAGT 1021 GGAAGAAGGG ATTTGTTCTT CATAAATCAA AGAGTGAAGA GGCTCATGCT GAAGATTCGG 1081 TTATGGACCA TCATTTCCGG AAGCCAGCAA ATGATATAAC GTCTCAGCTG GAGATCAATT 1141 TTGGAGACCT TGGCCGCCCA GGACGTGGCG GCAGGGGAGG ACGAGGTGGA CGTGGGCGTG 1201 GTGGGCGCCC AAACCGTGGC AGCAGGACCG ACAAGTCAAG TGCTTCTGCT CCTGATGTGG 1261 ATGACCCAGA GGCATTCCCA GCTCTGGCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCCAT 1441 GAGTAGGCCG AAGAGTCGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt