Construct: ORF TRCN0000469939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015756.1_s317c1
- Derived from:
- ccsbBroadEn_00028
- DNA Barcode:
- CTACTCCACACCTTTAATAACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH5 (128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469939
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128 | ADH5 | alcohol dehydrogenase 5 (cl... | NM_000671.4 | 100% | 100% | |
| 2 | mouse | 11532 | Adh5 | alcohol dehydrogenase 5 (cl... | NM_007410.3 | 88.5% | 93% | (many diffs) |
| 3 | mouse | 11532 | Adh5 | alcohol dehydrogenase 5 (cl... | NM_001288578.1 | 58.8% | 61.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaacgaggtt atcaagtgca aggctgcagt tgcttgggag gctggaaagc 121 ctctctccat agaggagata gaggtggcac ccccaaaggc tcatgaagtt cgaatcaaga 181 tcattgccac tgcggtttgc cacaccgatg cctataccct gagtggagct gatcctgagg 241 gttgttttcc agtgatcttg ggacatgaag gtgctggaat tgtggaaagt gttggtgagg 301 gagttactaa gctgaaggcg ggtgacactg tcatcccact ttacatccca cagtgtggag 361 aatgcaaatt ttgtctaaat cctaaaacta acctttgcca gaagataaga gtcactcaag 421 ggaaaggatt aatgccagat ggtaccagca gatttacttg caaaggaaag acaattttgc 481 attacatggg aaccagcaca ttttctgaat acacagttgt ggctgatatc tctgttgcta 541 aaatagatcc tttagcacct ttggataaag tctgccttct aggttgtggc atttcaaccg 601 gttatggtgc tgctgtgaac actgccaagt tggagcctgg ctctgtttgt gccgtctttg 661 gtctgggagg agtcggattg gcagttatca tgggctgtaa agtggctggt gcttcccgga 721 tcattggtgt ggacatcaat aaagataaat ttgcaagggc caaagagttt GGAGCCACTG 781 AATGTATTAA CCCTCAGGAT TTTAGTAAAC CCATCCAGGA AGTGCTCATT GAGATGACCG 841 ATGGAGGAGT GGACTATTCC TTTGAATGTA TTGGTAATGT GAAGGTCATG AGAGCAGCAC 901 TTGAGGCATG TCACAAGGGC TGGGGCGTCA GCGTCGTGGT TGGAGTAGCT GCTTCAGGTG 961 AAGAAATTGC CACTCGTCCA TTCCAGCTGG TAACAGGTCG CACATGGAAA GGCACTGCCT 1021 TTGGAGGATG GAAGAGTGTA GAAAGTGTCC CAAAGTTGGT GTCTGAATAT ATGTCCAAAA 1081 AGATAAAAGT TGATGAATTT GTGACTCACA ATCTGTCTTT TGATGAAATC AACAAAGCCT 1141 TTGAACTGAT GCATTCTGGA AAGAGCATTC GAACTGTTGT AAAGATTTAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGACTACTCC ACACCTTTAA TAACCTAACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt