Construct: ORF TRCN0000469957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011544.1_s317c1
- Derived from:
- ccsbBroadEn_16153
- DNA Barcode:
- AATTCTCTAGAACCCTGGCGGTCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM42 (287015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 287015 | TRIM42 | tripartite motif containing 42 | NM_152616.5 | 70.3% | 69.7% | (many diffs) |
| 2 | human | 287015 | TRIM42 | tripartite motif containing 42 | XM_011512740.3 | 69.9% | 69.3% | (many diffs) |
| 3 | human | 287015 | TRIM42 | tripartite motif containing 42 | XR_001740122.2 | 63.4% | (many diffs) | |
| 4 | human | 287015 | TRIM42 | tripartite motif containing 42 | XR_001740121.2 | 62.8% | (many diffs) | |
| 5 | mouse | 78911 | Trim42 | tripartite motif-containing 42 | NM_030219.2 | 60.8% | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1599
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaactgct atgtgcgttt gctgtccatg ttgtacatgg cagagatgtt 121 gtcctcagtt atgctcctgt ctgtgctgca agttcatctt cacctcagag cggaactgca 181 cctgcttccc ctgcccttac aaagatgagc ggaactgcca gttctgccac tgcacctgtt 241 ctgagagccc caactgccat tggtgttgct gctcttgggc caatgatccc aactgtaagt 301 gctgctgcac agccagcagc aatctcaact gctactacta tgagagccgc tgctgccgca 361 ataccatcat cactttccac aagggccgcc tcaggagcat ccatacctcc tccaagactg 421 ccctgcgcac tgggagcagc gatacccagg tggatgaagt aaagtcaata ccagccaaca 481 gtcacctggt gaaccacctc aattgcccca tgtgcagccg gctgcgcctg cactcattca 541 tgctgccctg caaccacagc ctgtgcgaga agtgcctgcg gcagctgcag aagcacgccg 601 aggtcaccga gaacttcttc atcctcatct gcccagtgtg cgaccgctcg cactgcatgc 661 cctacagcaa caagatgcag ctgcccgaga actacctgca cgggcgtctc accaagcgct 721 acatgcagga gcacggctac ctcaagtggc gctttgaccg ctcctccggg cccatcctct 781 gccaggtctg ccgcaacagg cgcatcgctt acaagcgctg catcacctgc cgcctcaacc 841 tgtgcaacga ctgcctcaag gccttccact cggatgtggc catgcaagac cacgtctttg 901 tggacaccag cgccgaggaa caggacgaga agatctgcat ccaccaccca tccagccgca 961 tcatcgagta ctgccgcaat gacaacaaat tgctctgcac cttctgcaag ttctctttcc 1021 acaatggcca cgacaccatt agcctcatcg acgcctgctc cgagagggcc gcctcactct 1081 tcagcgccat cgccaagttc aaagcagtcc gatatgaaat tgataatgac cTAATGGAAT 1141 TCAACATCTT AAAAAACAGC TTTAAAGCTG ACAAGGAGGC AAAGCGAAAA GAGATCAGAA 1201 ATGGCTTTCT CAAGTTGCGC AGCATTCTTC AGGAGAAAGA GAAGATCATC ATGGAGCAGA 1261 TAGAGAATCT AGAAGTGTCC AGGCAGAAGG AAATTGAAAA ATATGTGTAT GTTACAACCA 1321 TGAAAGTGAA CGAGATGGAT GGTCTGATCG CCTACTCCAA GGAAGCCCTG AAGGAGACTG 1381 GCCAGGTGGC ATTCCTGCAG TCAGCCAAGA TCCTGGTGGA CCAGATCGAG GACGGCATCC 1441 AGACCACCTA CAGGCCTGAC CCACAGCTCC GGCTGCACTC AATAAACTAC ATGCCCTTGG 1501 ACTTTGTTGA GCTTTCCAGT GCCATCCATG AGCTCTTCCC CACAGGGCCC AAGAAGGTAC 1561 GCTCCTCAGG GGACTCCCTG CCCTCCAGAA GCACTTCTTG CCAACTTTCT TGTACAAAGT 1621 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1681 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1741 CGAAATTCTC TAGAACCCTG GCGGTCAACG CGTTAAGTCg acaatcaacc tctggattac 1801 aaaatttgtg aaagatt