Construct: ORF TRCN0000470008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018239.1_s317c1
- Derived from:
- ccsbBroadEn_11955
- DNA Barcode:
- CCGAACACCCTGTAAAACACCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B12 (51144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51144 | HSD17B12 | hydroxysteroid 17-beta dehy... | NM_016142.3 | 30.7% | 30.1% | (many diffs) |
| 2 | human | 51144 | HSD17B12 | hydroxysteroid 17-beta dehy... | XM_017017881.1 | 18.6% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 360
- ORF length:
- 294
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA GAGCGCTCTC CCCGCCGCCG GCTTCCTGTA CTGGGTCGGC GCGGGCACCG 121 TGGCCTACCT AGCCCTGCGT ATTTCGTACT CGCTCTTCAC GGCCCTCCGG GTCTGGGGAG 181 TGGGGAATGA GGCGGGGGTC GGCCCGGGGC TCGGAGAATG GGCAGTTGTC ACAGGTAGTA 241 CTGATGGAAT TGGAAAATCA TATGCAGAAG AGTTAGCAAA GCATGGAATG AAGGTTGTCC 301 TTATCAGCAG ATCAAAGGAT AAACTTGACC AGGTTTCCAG TGAAATAAGC AATTATACTT 361 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGACCGAA CACCCTGTAA AACACCCGAA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt