Construct: ORF TRCN0000470035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003616.1_s317c1
- Derived from:
- ccsbBroadEn_10060
- DNA Barcode:
- CGAAGTCGGCAGCCGGCGTAGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GEN1 (348654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | NM_001130009.3 | 99.9% | 99.7% | 274T>A;929G>A |
| 2 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | NM_182625.5 | 99.9% | 99.7% | 274T>A;929G>A |
| 3 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | XM_005262613.4 | 99.9% | 99.7% | 274T>A;929G>A |
| 4 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | XM_006712005.3 | 99.9% | 99.7% | 274T>A;929G>A |
| 5 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | XM_011532820.2 | 99.9% | 99.7% | 274T>A;929G>A |
| 6 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | XM_011532821.2 | 99.9% | 99.7% | 274T>A;929G>A |
| 7 | human | 348654 | GEN1 | GEN1 Holliday junction 5' f... | XM_011532822.2 | 99.9% | 99.7% | 274T>A;929G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2793
- ORF length:
- 2724
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagtgaat gacttgtggc aaattttgga gcctgttaag caacacatcc 121 ccttgcgtaa tcttggtggg aaaaccattg cagttgatct gagtctctgg gtgtgtgagg 181 cacagacagt caaaaaaatg atgggcagcg tcatgaagcc ccacctcagg aacttatttt 241 ttcgtatctc atatttaaca caaatggatg taaaactggt atttgttatg gaaggggaac 301 caccaaagct gaaagctgat gtcataagca agaggaatca gactcggtat gggtcttctg 361 gaaaatcgtg gtctcagaaa acagggagat cacattttaa atcagtctta agagagtgcc 421 tccatatgct cgaatgctta ggaatcccct gggttcaggc tgctggggaa gctgaagcca 481 tgtgtgctta tctcaatgct ggtggtcatg tcgatggctg cctcaccaat gatggagata 541 ctttccttta tggggcccag actgtttaca ggaatttcac tatgaataca aaggacccac 601 atgttgactg ttacacaatg tcatctatca agagtaaact aggtttggat agagatgctc 661 tggttggatt agcaatactt cttggctgtg attatctccc aaagggagtc cctggagttg 721 gaaaagagca agcattaaaa cttatacaga ttttgaaagg gcaaagttta cttcagaggt 781 ttaatcggtg gaatgaaaca tcttgtaact ctagtccaca actgctagtc actaaaaaac 841 tggctcattg ttccgtatgt tcccatccag gttcacctaa ggatcatgaa cgtaatggat 901 gcagattatg taaaagtgat aaatattgtg agccacatga ctatgaatac tgctgtcctt 961 gtgagtggca ccgtacagaa catgataggc aactcaatga agtagagaac aatattaaga 1021 agaaagcttg ctgttgtgag ggattcccat tccatgaggt tattcaagaa ttccttttaa 1081 acaaggataa attggtgaag gttatcaggt accaaagacc tgatttgtta ttgtttcaga 1141 gatttactct tgaaaaaatg gagtggccca atcactatgc atgtgagaaa ttgctggtac 1201 ttttgaccca ttatgacatg atagaaagaa agcttggtag cagaaactct aatcaactac 1261 agccaattcg aattgttaag actcgaatca gaaatggagt tcattgtttt gaaatagaat 1321 gggaaaagcc tgaacattat gctatggaag ataaacaaca tggagaattt gctttattaa 1381 caattgagga agaatcattg tttgaagcag catatcctga gatcgttgct gtttaccaaa 1441 aacaaaagtt agaaattaaa gggaagaaac aaaaacgtat taagcctaaa gaaaacaatt 1501 tgccagaacc agatgaagta atgagctttc agtcacacat gactttaaaa cccacatgtg 1561 aaatctttca taagcagaat tccaagttaa attcggggat ttcccctgat cctacattac 1621 cacaggaatc tatttctgcc tcattgaata gcttgctttt acctaaaaat actccatgtt 1681 tgaatgcaca agaacagttc atgtcttctc taagaccttt ggctatacag caaattaaag 1741 ctgtcagtaa gtctctaatt tcagaatcta gtcaacccaa tacctcatct cataatatat 1801 ccgtgattgc tgatctacac ttgagcacta ttgactggga aggtacttct tttagtaatt 1861 ctccagctat tcaaaggaat actttttctc atgatttaaa atcagaagtt gaatcagagc 1921 tatcagccat ccctgatggc tttgaaaata tcccagaaca actgtcctgt gaatcagaaa 1981 ggtacactgc aaacataaag aaagtgttgg atgaggattc tgatgggatt agtcctgaag 2041 agcatctact ttctggcatt actgatttat gtcttcagga tttgccttta aaggaacgaa 2101 tatttacaaa attatcatat cctcaggata atctacaacc agatgtcaac ctgaaaactt 2161 tgtccatact tagtgtaaaa gaatcttgta ttgctaacag tggttctgat tgtacatcac 2221 atctttcaaa ggatcttcca ggaattccct tgcaaaatga atccagagac tctaaaattc 2281 taaaaggaga ccagctgctt caagaagact ataaagtcaa tacttctgtc ccttattctg 2341 tcagtaacac agtggtaaag accTGCAATG TTAGACCACC AAATACTGCT TTAGATCATA 2401 GTAGAAAAGT TGATATGCAA ACCACTCGGA AAATTTTAAT GAAGAAGAGT GTTTGCCTTG 2461 ACAGACATTC CTCTGATGAA CAAAGTGCCC CAGTGTTTGG GAAAGCTAAG TACACAACTC 2521 AAAGAATGAA GCACAGTTCT CAAAAGCATA ATTCATCCCA TTTCAAAGAA AGTGGCCATA 2581 ACAAGTTGAG TAGCCCTAAG ATACATATTA AAGAAACTGA ACAGTGTGTC AGATCTTATG 2641 AAACAGCTGA AAATGAAGAA AGCTGTTTCC CAGATTCAAC AAAAAGTTCT CTGAGTTCTC 2701 TACAATGTCA TAAGAAAGAA AACAACTCTG GTACTTGTTT GGATAGCCCT CTTCCTTTAC 2761 GCCAGAGATT AAAACTAAGA TTCCAAAGCA CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2821 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2881 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 2941 AAGTCGGCAG CCGGCGTAGC TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 3001 ttgtgaaaga tt