Construct: ORF TRCN0000470047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015454.1_s317c1
- Derived from:
- ccsbBroadEn_06028
- DNA Barcode:
- AACGGGGGACCGTCTTTCGAGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPE (1363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1363 | CPE | carboxypeptidase E | NM_001873.4 | 99.9% | 100% | 801C>T |
| 2 | mouse | 12876 | Cpe | carboxypeptidase E | NM_013494.4 | 88.5% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggcgaggg ggcagcgcgc tgctggctct gtgcggggca ctggctgcct 121 gcgggtggct cctgggcgcc gaagcccagg agcccggggc gcccgcggcg ggcatgaggc 181 ggcgccggcg gctgcagcaa gaggacggca tctccttcga gtaccaccgc taccccgagc 241 tgcgcgaggc gctcgtgtcc gtgtggctgc agtgcaccgc catcagcagg atttacacgg 301 tggggcgcag cttcgagggc cgggagctcc tggtcatcga gctgtccgac aaccctggcg 361 tccatgagcc tggtgagcct gaatttaaat acattgggaa tatgcatggg aatgaggctg 421 ttggacgaga actgctcatt ttcttggccc agtacctatg caacgaatac cagaagggga 481 acgagacaat tgtcaacctg atccacagta cccgcattca catcatgcct tccctgaacc 541 cagatggctt tgagaaggca gcgtctcagc ctggtgaact caaggactgg tttgtgggtc 601 gaagcaatgc ccagggaata gatctgaacc ggaactttcc agacctggat aggatagtgt 661 acgtgaatga gaaagaaggt ggtccaaata atcatctgtt gaaaaatatg aagaaaattg 721 tggatcaaaa cacaaagctt gctcctgaga ccaaggctgt cattcattgg attatggata 781 ttccttttgt gctttctgcc aatctccatg gaggagacct tgtggccaat tatccatatg 841 atgagacgcg gagtggtagt gctcatgaat acagctcctc cccagatgac gccattttcc 901 aaagcttggc ccgggcatac tcttctttca acccggccat gtctgacccc aatcggccac 961 catgtcgcaa gaatgatgat gacagcagct ttgtagatgg aaccaccaac ggtggtgctt 1021 ggtacagcgt acctggaggg atgcaagact tcaattaccT TAGCAGCAAC TGTTTTGAGA 1081 TCACCGTGGA GCTTAGCTGT GAGAAGTTCC CACCTGAAGA GACTCTGAAG ACCTACTGGG 1141 AGGATAACAA AAACTCCCTC ATTAGCTACC TTGAGCAGAT ACACCGAGGA GTTAAAGGAT 1201 TTGTCCGAGA CCTTCAAGGT AACCCAATTG CGAATGCCAC CATCTCCGTG GAAGGAATAG 1261 ACCACGATGT TACATCCGCA AAGGATGGTG ATTACTGGAG ATTGCTTATA CCTGGAAACT 1321 ATAAACTTAC AGCCTCAGCT CCAGGCTATC TGGCAATAAC AAAGAAAGTG GCAGTTCCTT 1381 ACAGCCCTGC TGCTGGGGTT GATTTTGAAC TGGAGTCATT TTCTGAAAGG AAAGAAGAGG 1441 AGAAGGAAGA ATTGATGGAA TGGTGGAAAA TGATGTCAGA AACTTTAAAT TTTTACCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAA ACGGGGGACC GTCTTTCGAG AGCACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att