Construct: ORF TRCN0000470049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014107.1_s317c1
- Derived from:
- ccsbBroadEn_03304
- DNA Barcode:
- CGCTCAACAGTTGTCCCTGGCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX41 (51428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470049
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51428 | DDX41 | DEAD-box helicase 41 | NM_016222.4 | 100% | 100% | |
| 2 | human | 51428 | DDX41 | DEAD-box helicase 41 | XM_024446109.1 | 80% | 80% | (many diffs) |
| 3 | human | 51428 | DDX41 | DEAD-box helicase 41 | NM_001321732.2 | 79.7% | 79.7% | 0_1ins378 |
| 4 | human | 51428 | DDX41 | DEAD-box helicase 41 | NM_001321830.2 | 79.7% | 79.7% | 0_1ins378 |
| 5 | mouse | 72935 | Ddx41 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_134059.2 | 89.3% | 98.8% | (many diffs) |
| 6 | mouse | 72935 | Ddx41 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017315611.1 | 69% | 76.8% | (many diffs) |
| 7 | mouse | 72935 | Ddx41 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_017315612.1 | 65.8% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1932
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagtcggaa cccgaacgga agcgggctcg caccgacgag gtgcctgccg 121 gaggaagccg ctccgaggcg gaagatgagg acgacgagga ctacgtgccc tatgtgccgt 181 tacggcagcg ccggcagcta ctgctccaga agctgctgca gcgaagacgc aagggagctg 241 cggaggaaga gcagcaggac agcggtagtg aaccccgggg agatgaggac gacatcccgc 301 taggccctca gtccaacgtc agcctcctgg atcagcacca gcaccttaaa gagaaggctg 361 aagcgcgcaa agagtctgcc aaggagaagc agctgaagga agaagagaag atcctggaga 421 gtgttgccga gggccgagca ttgatgtcag tgaaggagat ggctaagggc attacgtatg 481 atgaccccat caaaaccagc tggactccac cccgttatgt tctgagcatg tctgaagagc 541 gacatgagcg cgtgcggaag aaataccaca tcctggtgga gggagacggt atcccaccac 601 ccatcaagag cttcaaggaa atgaagtttc ctgcagccat cctgagaggc ctgaagaaga 661 aaggcattca ccacccaaca cccattcaga tccagggcat ccccaccatt ctatctggcc 721 gtgacatgat aggcatcgct ttcacgggtt caggcaagac actggtgttc acgttgcccg 781 tcatcatgtt ctgcctggaa caagagaaga ggttaccctt ctcaaagcgc gaggggccct 841 atggactcat catctgcccc tcgcgggagc tggcccggca gacccatggc atcctggagt 901 actactgccg cctgctgcag gaggacagct caccactcct gcgctgcgcc ctctgcattg 961 ggggcatgtc cgtgaaagag cagatggaga ccatccgaca cggtgtacac atgatggtgg 1021 ccaccccggg gcgcctcatg gatttgctgc agaagaagat ggtcagccta gacatctgtc 1081 gctacctggc cctggacgag gctgaccgca tgatcgacat gggcttcgag ggtgacatcc 1141 gtaccatctt ctcctacttc aagggccagc gacagaccct gctcttcagt gccaccatgc 1201 cgaagaagat tcagaacttt gctaagagtg cccttgtaaa gcctgtgacc atcaatgtgg 1261 ggcgcgctgg ggctgccagc ctggatgtca tccaggaggt agaatatgtg aaggaggagg 1321 ccaagatggt gtacctgctc gagtgcctgc agaagacacc cccgcctgta ctcatctttg 1381 cagagaagaa ggcagacgtg gacgccatcc acgagtacct gctgctcaag ggggttgagg 1441 ccgtagccat ccatggggGC AAAGACCAGG AGGAACGGAC TAAGGCCATC GAGGCATTCC 1501 GGGAGGGCAA GAAGGATGTC CTAGTAGCCA CAGACGTTGC CTCCAAGGGC CTGGACTTCC 1561 CTGCCATCCA GCACGTCATC AATTATGACA TGCCAGAGGA GATTGAGAAC TATGTACACC 1621 GGATTGGCCG CACCGGGCGC TCGGGAAACA CAGGCATCGC CACTACCTTC ATCAACAAAG 1681 CGTGTGATGA GTCAGTGCTG ATGGACCTCA AAGCGCTGCT GCTAGAAGCC AAGCAGAAGG 1741 TGCCGCCCGT GCTGCAGGTG CTGCATTGCG GGGATGAGTC CATGCTGGAC ATTGGAGGAG 1801 AGCGCGGCTG TGCCTTCTGC GGGGGCCTGG GTCATCGGAT CACTGACTGC CCCAAACTCG 1861 AGGCTATGCA GACCAAGCAG GTCAGCAACA TCGGTCGCAA GGACTACCTG GCCCACAGCT 1921 CCATGGACTT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGC TCAACAGTTG TCCCTGGCAT 2101 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t